Safety Aspect of Enterococcus faecium FL31 Strain and Antibacterial Mechanism of Its Hydroxylated Bacteriocin BacFL31 against Listeria monocytogenes
Joint Authors
Smaoui, Slim
Mtibaa, Ahlem Chakchouk
Sellem, Imen
Kamoun, Yosra
Karray-Rebai, Ines
Mellouli, Lotfi
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In previous work we have isolated and identified a new strain called Enterococcus faecium FL31.
The active compound secreted by this strain, “BacFL31”, has been purified and characterized.
In the present study, safety aspect, assessed by microbiological and molecular tests, demonstrated that Enterococcus faecium FL31 was susceptible to relevant antibiotics, free of hemolytic, gelatinase, DNase, and lipase activities.
In addition, it did not harbor virulence and antibiotic resistance genes.
Combined SYTOX Green dye and UV-absorbing experiments, along with released extracellular potassium and transmembrane electrical potential measurements, showed that pure BacFL31 at a concentration of 1×MIC (50 μg/mL) could damage cytoplasmic membrane of the pathogen Listeria monocytogenes ATCC19117.
The same concentration causes the leakage of its intracellular constituents and leads to the destruction of this pathogenic microorganism.
In summary, this work reflected characteristics of Enterococcus faecium FL31 strain and its bacteriocin in terms of functional and safety perspectives.
American Psychological Association (APA)
Mtibaa, Ahlem Chakchouk& Sellem, Imen& Kamoun, Yosra& Smaoui, Slim& Karray-Rebai, Ines& Mellouli, Lotfi. 2018. Safety Aspect of Enterococcus faecium FL31 Strain and Antibacterial Mechanism of Its Hydroxylated Bacteriocin BacFL31 against Listeria monocytogenes. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1127212
Modern Language Association (MLA)
Mtibaa, Ahlem Chakchouk…[et al.]. Safety Aspect of Enterococcus faecium FL31 Strain and Antibacterial Mechanism of Its Hydroxylated Bacteriocin BacFL31 against Listeria monocytogenes. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1127212
American Medical Association (AMA)
Mtibaa, Ahlem Chakchouk& Sellem, Imen& Kamoun, Yosra& Smaoui, Slim& Karray-Rebai, Ines& Mellouli, Lotfi. Safety Aspect of Enterococcus faecium FL31 Strain and Antibacterial Mechanism of Its Hydroxylated Bacteriocin BacFL31 against Listeria monocytogenes. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1127212
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127212