MicroRNA-205-5p Targets HMGB1 to Suppress Inflammatory Responses during Lung Injury after Hip Fracture
Joint Authors
Yu, Xiaojie
Chen, Xiaobin
Sun, Tiansheng
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-28
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Hip fracture is the most common type of injury in elderly people and is associated with a high incidence of complications and risk of mortality.
In these patients, subsequent pulmonary infection can contribute to the development of an acute lung injury, a consequence of the systemic inflammatory response induced by hip fracture.
Although the crucial role of microRNAs (miRNAs) in inflammatory responses has been established, the functions of miRNAs in the inflammatory responses associated with lung injury after hip fracture remain poorly understood.
In this study, we explored the potential role of miR-205-5p in lung injury after hip fracture in an in vivo hip fracture model and in vitro cultures of human pulmonary alveolar epithelial cells (HPAEpiC).
An analysis of clinical serum samples revealed increased levels of miR-205-5p and high mobility group box 1 (HMGB1) after hip fracture.
A bioinformatics analysis and dual-luciferase reporter assay identified HMGB1 as a potential target of miR-205-5p.
The overexpression of miR-205-5p clearly reduced the expression of HMGB1 and inhibited NF-κB signaling, apoptosis, and proinflammatory cytokine production while enabling continued cell proliferation.
Our results demonstrate that the upregulation of miR-205-5p suppresses inflammatory responses and promotes cell viability and proliferation by selectively targeting HMGB1 in the context of lung injury after hip fracture.
Therefore, miR-205-5p may be an alternative target of therapeutic strategies for lung injury after hip fracture.
American Psychological Association (APA)
Yu, Xiaojie& Chen, Xiaobin& Sun, Tiansheng. 2019. MicroRNA-205-5p Targets HMGB1 to Suppress Inflammatory Responses during Lung Injury after Hip Fracture. BioMed Research International،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1127221
Modern Language Association (MLA)
Yu, Xiaojie…[et al.]. MicroRNA-205-5p Targets HMGB1 to Suppress Inflammatory Responses during Lung Injury after Hip Fracture. BioMed Research International No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1127221
American Medical Association (AMA)
Yu, Xiaojie& Chen, Xiaobin& Sun, Tiansheng. MicroRNA-205-5p Targets HMGB1 to Suppress Inflammatory Responses during Lung Injury after Hip Fracture. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1127221
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127221