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Statistical Optimization of Culture Conditions for Protein Production by a Newly Isolated Morchella fluvialis
Joint Authors
Rahgo, Zahra
Samadlouie, Hamid reza
Mojerlou, Shideh
Jahanbin, Kambiz
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Morchella fungi are considered a good source of protein.
The ITS region was used to identify Morchella isolated in the northern region of Iran.
The isolated fungus was very similar to Morchella fluvialis.
M.
fluvialis was first isolated in Iran.
Dried biomass of M.
fluvialis contained 9% lipids and 50% polysaccharides.
Fatty acid profiles of lipids of M.
fluvialis are mainly made up of linoleic acid (C18:2) (62%), followed by palmitic acid (C16:0) (12%).
Testosterone (TS) was also detected (0.732 ng/dry weight biomass (DWB)) in the hormone profile of this new isolated species.
Then, various protein and carbon sources as variable factors were applied to identify the key substrates, which stimulated protein production using the one-factor-at-a-time method.
Key substrates (glucose and soybean) were statistically analyzed to determine the optimum content of the protein and DWB accumulation using response surface methods.
The highest protein content (38% DWB) was obtained in the medium containing 80 g/l glucose and 40 g/l soybean powder.
Total nutritionally indispensable amino acids and conditionally indispensable amino acids constitute 55.7% crude protein.
That is to say, these adequate quantities of essential amino acids in the protein of M.
fluvialis make it a good and promising source of essential amino acids for human diet.
American Psychological Association (APA)
Rahgo, Zahra& Samadlouie, Hamid reza& Mojerlou, Shideh& Jahanbin, Kambiz. 2019. Statistical Optimization of Culture Conditions for Protein Production by a Newly Isolated Morchella fluvialis. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1127237
Modern Language Association (MLA)
Rahgo, Zahra…[et al.]. Statistical Optimization of Culture Conditions for Protein Production by a Newly Isolated Morchella fluvialis. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1127237
American Medical Association (AMA)
Rahgo, Zahra& Samadlouie, Hamid reza& Mojerlou, Shideh& Jahanbin, Kambiz. Statistical Optimization of Culture Conditions for Protein Production by a Newly Isolated Morchella fluvialis. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1127237
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127237