![](/images/graphics-bg.png)
Assessment of Airborne Bacterial and Fungal Communities in Selected Areas of Teaching Hospital, Kandy, Sri Lanka
Joint Authors
Magana-Arachchi, Dhammika N.
Sivagnanasundaram, Premina
Amarasekara, R. W. K.
Madegedara, R. M. D.
Ekanayake, Anuradha
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Nosocomial infections, in lay term known as hospital acquired infections, are caused mainly by airborne pathogens found in healthcare facilities and their surroundings.
The aim of this study was to quantify and identify bacteria and fungi in a hospital, which is an understudied area of air quality in Sri Lanka.
Air samples were collected in agar medium and petri plates containing sterile filter papers.
The number of culturable and total airborne microorganisms was estimated by manual counting and fluorescent microscopy, respectively.
The morphologically distant bacteria and fungi were identified by DNA sequencing.
The statistical analysis revealed significant variances between studied sites (p < 0.05) where Outpatients Department and Respiratory Unit showed higher levels of airborne microbial load.
Culturable microbial count was higher at noon (hospital visiting hours) compared to other sampling periods (after hospital visiting hours) within the hospital.
Total count of airborne microbes was found to be the highest during the afternoon.
The most sensitive zones such as Operating Theatre and Intensive Care Unit showed considerably higher counts of airborne microbes.
Identification by molecular means revealed the presence of human pathogens in the hospital air including Bacillus sp, Micrococcus sp, Pseudomonas sp, Staphylococcu ssp, Exiguobacterium sp, Enterobacter sp, Escherichia sp, Sphingomonas sp, Massilia sp, Kocuria sp, Fusarium sp, and Aspergillus sp.
In conclusion, the results from this study indicate that the hospital air was generally contaminated.
Therefore, the implementation of proactive policies and strategies are needed to monitor hospital air quality in sensitive zones as well as other areas of the hospitals.
American Psychological Association (APA)
Sivagnanasundaram, Premina& Amarasekara, R. W. K.& Madegedara, R. M. D.& Ekanayake, Anuradha& Magana-Arachchi, Dhammika N.. 2019. Assessment of Airborne Bacterial and Fungal Communities in Selected Areas of Teaching Hospital, Kandy, Sri Lanka. BioMed Research International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1127268
Modern Language Association (MLA)
Sivagnanasundaram, Premina…[et al.]. Assessment of Airborne Bacterial and Fungal Communities in Selected Areas of Teaching Hospital, Kandy, Sri Lanka. BioMed Research International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1127268
American Medical Association (AMA)
Sivagnanasundaram, Premina& Amarasekara, R. W. K.& Madegedara, R. M. D.& Ekanayake, Anuradha& Magana-Arachchi, Dhammika N.. Assessment of Airborne Bacterial and Fungal Communities in Selected Areas of Teaching Hospital, Kandy, Sri Lanka. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1127268
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127268