Immobilization of a Laccase2,2’-azino-bis-(3-ethylbenzothiazoline)‎-6-sulfonic Acid System to Layered Double HydroxideAlginate Biohybrid Beads for Biodegradation of Malachite Green Dye

Abstract EN

The application of laccase-mediator-based catalysis is limited owing to the high cost of laccases and mediators and the potential toxicity of free mediators.

Here, a novel biocatalyst (Im-LMS) was fabricated by immobilizing both laccase and a mediator (2,2’-azino-bis-[3-ethylbenzothiazoline]-6-sulfonic acid) on layered double hydroxide/alginate biohybrid beads.

The catalytic activity of Im-LMS was evaluated for dye decolorization using malachite green.

The decolorization yields of malachite green by Im-LMS and the free laccase-mediator system were 92% within 120 min and 90% within 90 min.

Malachite green solution was detoxified completely after biodegradation by Im-LMS.

Following eight reuse cycles of Im-LMS for dye treatment, a decolorization yield of 79% was obtained.

The activity of Im-LMS was almost completely stable after being stored for 10 days.

The recyclability and stability of Im-LMS will be helpful for reducing the running cost and potential toxicity associated with mediators to facilitate practical applications.