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Oncogenic N-Ras Stimulates SRF-Mediated Transactivation via H3 Acetylation at Lysine 9
Joint Authors
Yi, Sun-Ju
Hwang, Seong Yun
Oh, Myung-Ju
Ryu, Hojin
Rhee, Sung-Keun
Jhun, Byung H.
Kim, Kyunghwan
Kim, Yang-Hoon
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Signal transduction pathways regulate the gene expression by altering chromatin dynamics in response to mitogens.
Ras proteins are key regulators linking extracellular stimuli to a diverse range of biological responses associated with gene regulation.
In mammals, the three ras genes encode four Ras protein isoforms: H-Ras, K-Ras4A, K-Ras4B, and N-Ras.
Although emerging evidence suggests that Ras isoforms differentially regulate gene expressions and are functionally nonredundant, the mechanisms underlying Ras specificity and Ras signaling effects on gene expression remain unclear.
Here, we show that oncogenic N-Ras acts as the most potent regulator of SRF-, NF-κB-, and AP-1-dependent transcription.
N-Ras-RGL2 axis is a distinct signaling pathway for SRF target gene expression such as Egr1 and JunB, as RGL2 Ras binding domain (RBD) significantly impaired oncogenic N-Ras-induced SRE activation.
By monitoring the effect of Ras isoforms upon the change of global histone modifications in oncogenic Ras-overexpressed cells, we discovered that oncogenic N-Ras elevates H3K9ac/H3K23ac levels globally in the chromatin context.
Importantly, chromatin immunoprecipitation (ChIP) assays revealed that H3K9ac is significantly enriched at the promoter and coding regions of Egr1 and JunB.
Collectively, our findings define an undocumented role of N-Ras in modulating of H3 acetylation and in gene regulation.
American Psychological Association (APA)
Yi, Sun-Ju& Hwang, Seong Yun& Oh, Myung-Ju& Kim, Yang-Hoon& Ryu, Hojin& Rhee, Sung-Keun…[et al.]. 2018. Oncogenic N-Ras Stimulates SRF-Mediated Transactivation via H3 Acetylation at Lysine 9. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1127335
Modern Language Association (MLA)
Yi, Sun-Ju…[et al.]. Oncogenic N-Ras Stimulates SRF-Mediated Transactivation via H3 Acetylation at Lysine 9. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1127335
American Medical Association (AMA)
Yi, Sun-Ju& Hwang, Seong Yun& Oh, Myung-Ju& Kim, Yang-Hoon& Ryu, Hojin& Rhee, Sung-Keun…[et al.]. Oncogenic N-Ras Stimulates SRF-Mediated Transactivation via H3 Acetylation at Lysine 9. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1127335
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127335