Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats
Joint Authors
Merino, O.
Sánchez, R.
Gregorio, B. M.
Sampaio, F. J.
Risopatrón, J.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-25
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Obesity has adverse effects on male fertility and usually is diagnosed with a prevalence of vitamin D deficiency (VD-).
Discussion on the impact of obesity/VD- on sperm function has been limited.
This study analyzed the effects of diet-induced obesity/VD- on viability and plasma membrane integrity (PMI), superoxide anion (O2-) level, and DNA fragmentation (DNAfrag) in sperm Sprague-Dawley rats.
The males were randomized into four groups and fed for a period of 12 weeks: G1: control diet with vitamin D (C/VD+), G2: control diet without vitamin D (C/VD-), G3: high-fat diet with vitamin D (HF/VD+), and G4: high-fat diet without vitamin D (HF/VD-).
Sperm function parameters were analyzed by flow cytometry.
PMI percentages and O2- levels were not affected by any of the diets.
DNA fragmentation was increasing significantly (p<0.05) in the spermatozoa of animals with diets vitamin D deficient (G2) and diet-induced obesity (G4).
Our results allow us to point out that diet-induced obesity and VD- produce greater damage in DNA sperm of rats.
The use of nutraceuticals containing vitamin D could be reducing the risk of fragmentation of DNA in spermatozoa.
American Psychological Association (APA)
Merino, O.& Sánchez, R.& Gregorio, B. M.& Sampaio, F. J.& Risopatrón, J.. 2018. Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats. BioMed Research International،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1127344
Modern Language Association (MLA)
Merino, O.…[et al.]. Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats. BioMed Research International No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1127344
American Medical Association (AMA)
Merino, O.& Sánchez, R.& Gregorio, B. M.& Sampaio, F. J.& Risopatrón, J.. Effects of Diet-Induced Obesity and Deficient in Vitamin D on Spermatozoa Function and DNA Integrity in Sprague-Dawley Rats. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1127344
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127344