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Reduced β2GPI Inhibiting Glomerular Mesangial Cells VEGF-NO Axis Uncoupling Induced by High Glucose
Joint Authors
Yu, Pei
Saijun, Zhou
Xin, Li
Jie, Wang
Shumin, Xiao
Shuaihui, Liu
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
VEGF-NO axis uncoupling is an important pathogenesis for DN.
Reduced β2GPI could play a part in VEGF signaling pathway and has a protective effect on diabetic vascular disease.
This study investigates the effect of reduced β2GPI on glomerular mesangial cells VEGF-NO axis uncoupling induced by high glucose.
Compared to control group, glomerular mesangial cell line HBZY-1 cells treated with high glucose expressed higher levels of VEGF mRNA and protein and produced more ROS but less NO.
The related proteins related to VEGF-NO axis were assayed.
High glucose could significantly increase the expression of the level of VEGFR2 and obviously increase phosphorylation of Akt and eNOS but significantly decrease the expression of GTP cyclohydrolase 1 (GCH-1), reducing the production of eNOS dimer.
Both β2GPI and reduced β2GPI partly reverse these effects caused by high glucose.
Reduced β2GPI had stronger effect than β2GPI.
GCH-1 is the speed limit of tetrahydrobiopterin (BH4) synthesis enzyme.
As the key part of eNOS cofactors, BH4 could partly restore eNOS dimer induced by high glucose.
Our results indicated that high glucose could interfere with eNOS dimer formation.
β2GPI and reduced β2GPI can partly reverse the VEGF-NO axis uncoupling by restoring the GCH-1 expression level and then promote eNOS dimer formation.
American Psychological Association (APA)
Saijun, Zhou& Xin, Li& Jie, Wang& Shumin, Xiao& Shuaihui, Liu& Yu, Pei. 2018. Reduced β2GPI Inhibiting Glomerular Mesangial Cells VEGF-NO Axis Uncoupling Induced by High Glucose. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1127349
Modern Language Association (MLA)
Saijun, Zhou…[et al.]. Reduced β2GPI Inhibiting Glomerular Mesangial Cells VEGF-NO Axis Uncoupling Induced by High Glucose. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1127349
American Medical Association (AMA)
Saijun, Zhou& Xin, Li& Jie, Wang& Shumin, Xiao& Shuaihui, Liu& Yu, Pei. Reduced β2GPI Inhibiting Glomerular Mesangial Cells VEGF-NO Axis Uncoupling Induced by High Glucose. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1127349
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127349