A Cross-Sectional Study of Entamoeba histolyticadisparmoshkovskii Complex in Salvador, Bahia, Brazil
Joint Authors
Teixeira, Marcia C. A.
Pacheco, Flavia T. F.
Soares, Neci M.
Azevedo, Helen C.
de Souza, Joelma N.
Del-Rei, Rodrigo P.
Santos, Fred L. N.
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Epidemiological studies on species-specific Entamoeba infections are scarce due to the morphological similarity of pathogenic Entamoeba histolytica and nonpathogenic E.
dispar and E.
moshkovskii.
The diagnosis of E.
histolytica is frequently based on coproantigen (E.
histolytica-Gal/GalNAc lectin specific) detection by immunoassays.
However, specific E.
histolytica-lectin is not expressed in cysts, which are eliminated by asymptomatic individuals leading to false-negative results and an underestimation of amebiasis prevalence.
Molecular techniques based on the amplification of parasite DNA have been shown to be a highly sensitive and specific method that allows the detection of different Entamoeba species.
This study aimed to assess the frequency of the species from E.
histolytica/dispar/moshkovskii complex by molecular and immunological techniques in individuals attended at a public health system in Salvador-Bahia, Brazil.
A cross-sectional study involving 55,218 individuals was carried out.
The diagnosis was based on microscopy revealing E.
histolytica/dispar/moshkovskii complex.
The species differentiation was performed by E.
histolytica-specific antigen, serological evaluation and by molecular technique.
The overall prevalence of E.
histolytica/dispar/moshkovskii complex determined by microscopy was approximately 0.49% (273/55,218).
E.
histolytica-specific antigen detection and molecular characterization returned 100% negativity for E.
histolytica.
However, serological evaluation returned an 8.9% positivity (8/90).
In the stool samples analysed by PCR, it was not possible to identify E.
histolytica and E.
moshkovskii, although circulating IgG anti-E.
histolytica has been detected.
American Psychological Association (APA)
Soares, Neci M.& Azevedo, Helen C.& Pacheco, Flavia T. F.& de Souza, Joelma N.& Del-Rei, Rodrigo P.& Teixeira, Marcia C. A.…[et al.]. 2019. A Cross-Sectional Study of Entamoeba histolyticadisparmoshkovskii Complex in Salvador, Bahia, Brazil. BioMed Research International،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1127366
Modern Language Association (MLA)
Soares, Neci M.…[et al.]. A Cross-Sectional Study of Entamoeba histolyticadisparmoshkovskii Complex in Salvador, Bahia, Brazil. BioMed Research International No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1127366
American Medical Association (AMA)
Soares, Neci M.& Azevedo, Helen C.& Pacheco, Flavia T. F.& de Souza, Joelma N.& Del-Rei, Rodrigo P.& Teixeira, Marcia C. A.…[et al.]. A Cross-Sectional Study of Entamoeba histolyticadisparmoshkovskii Complex in Salvador, Bahia, Brazil. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1127366
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127366