LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
Joint Authors
Miljkovic, Marija
Malesevic, Milka
Filipic, Brankica
Vukotic, Goran
Kojic, Milan
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Restriction enzymes are the main defence system against foreign DNA, in charge of preserving genome integrity.
Lactococcus raffinolactis BGTRK10-1 expresses LraI Type II restriction-modification enzyme, whose activity is similar to that shown for EcoRI; LraI methyltransferase protects DNA from EcoRI cleavage.
The gene encoding LraI endonuclease was cloned and overexpressed in E.
coli.
Purified enzyme showed the highest specific activity at lower temperatures (between 13°C and 37°C) and was stable after storage at −20°C in 50% glycerol.
The concentration of monovalent ions in the reaction buffer required for optimal activity of LraI restriction enzyme was 100 mM or higher.
The recognition and cleavage sequence for LraI restriction enzyme was determined as 5′-G/AATTC-3′, indicating that LraI restriction enzyme is an isoschizomer of EcoRI.
In the reaction buffer with a lower salt concentration, LraI exhibits star activity and specifically recognizes and cuts another alternative sequence 5′-A/AATTC-3′, leaving the same sticky ends on fragments as EcoRI, which makes them clonable into a linearized vector.
Phylogenetic analysis based on sequence alignment pointed out the common origin of LraI restriction-modification system with previously described EcoRI-like restriction-modification systems.
American Psychological Association (APA)
Miljkovic, Marija& Malesevic, Milka& Filipic, Brankica& Vukotic, Goran& Kojic, Milan. 2018. LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1127395
Modern Language Association (MLA)
Miljkovic, Marija…[et al.]. LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1127395
American Medical Association (AMA)
Miljkovic, Marija& Malesevic, Milka& Filipic, Brankica& Vukotic, Goran& Kojic, Milan. LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1127395
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127395