Cox-2 Negatively Affects the Protective Role of Propofol against HypoxiaReoxygenation Induced Cardiomyocytes Apoptosis through Suppressing Akt Signaling
Joint Authors
Ma, Kangmu
Qiu, Jiapei
Zhou, Mi
Yang, Yang
Ye, Xiaofeng
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-16
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Nowadays, the prevention of severe myocardium injury resulting from myocardial ischemia/reperfusion injury (I/R) has been recognized as an important subject in the field of ischemic heart disease.
In this study, H9c2 cardiomyocytes were exposed to cycles of hypoxia/reoxygenation (H/R) to mimic myocardial I/R injury.
Western blot analysis and qRT-PCR were performed to detect the expression of Cox-2, Akt and p-Akt.
Cell viability, LDH release and activity of Caspase-3 were assessed to determine the protective effect of propofol.
The results proved that the protective effect of propofol for H/R challenged cardiomyocytes was associated with Akt phosphorylation.
We also revealed that treatment of propofol suppressed the expression of Cox-2 in cardiomyocytes which was up-regulated after H/R treatment.
Conversely, the over-expression of Cox-2 inhibited Akt phosphorylation while enhancing cardiomyocytes apoptosis.
Interestingly, Akt activator exhibited similar protective effect with propofol and could diminish the influences brought by over-expression of Cox-2.
Thus, it could be concluded that Cox-2 negatively affects the protective effect of propofol against hypoxia/reoxygenation induced cardiomyocyte apoptosis by suppressing Akt phosphorylation.
American Psychological Association (APA)
Ma, Kangmu& Qiu, Jiapei& Zhou, Mi& Yang, Yang& Ye, Xiaofeng. 2019. Cox-2 Negatively Affects the Protective Role of Propofol against HypoxiaReoxygenation Induced Cardiomyocytes Apoptosis through Suppressing Akt Signaling. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1127410
Modern Language Association (MLA)
Ma, Kangmu…[et al.]. Cox-2 Negatively Affects the Protective Role of Propofol against HypoxiaReoxygenation Induced Cardiomyocytes Apoptosis through Suppressing Akt Signaling. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1127410
American Medical Association (AMA)
Ma, Kangmu& Qiu, Jiapei& Zhou, Mi& Yang, Yang& Ye, Xiaofeng. Cox-2 Negatively Affects the Protective Role of Propofol against HypoxiaReoxygenation Induced Cardiomyocytes Apoptosis through Suppressing Akt Signaling. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1127410
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127410