Reperfusion Activates AP-1 and Heat Shock Response in Donor Kidney Parenchyma after Warm Ischemia
Joint Authors
Reznik, Alexandr
Plotnikova, Olga
Skvortsov, Andrey
Skoblov, Mikhail
Reznik, Oleg
Baranova, Ancha
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-16
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Utilization of kidneys from extended criteria donors leads to an increase in average warm ischemia time (WIT), which is associated with larger degrees of ischemia-reperfusion injury (IRI).
Kidney resuscitation by extracorporeal perfusion in situ allows up to 60 minutes of asystole after the circulatory death.
Molecular studies of kidney grafts from human donors with critically expanded WIT are warranted.
Transcriptomes of two human kidneys from two different donors were profiled after 35-45 minutes of WIT and after 120 minutes of normothermic perfusion and compared.
Baseline gene expression patterns in ischemic grafts display substantial intrinsic differences.
IRI does not lead to substantial change in overall transcription landscape but activates a highly connected protein network with hubs centered on Jun/Fos/ATF transcription factors and HSP1A/HSPA5 heat shock proteins.
This response is regulated by positive feedback.
IRI networks are enriched in soluble proteins and biofluids assayable substances, thus, indicating feasibility of the longitudinal, minimally invasive assessment in vivo.
Mapping of IRI related molecules in ischemic and reperfused kidneys provides a rationale for possible organ conditioning during machine assisted ex vivo normothermic perfusion.
A study of natural diversity of the transcriptional landscapes in presumably normal, transplantation-suitable human organs is warranted.
American Psychological Association (APA)
Reznik, Alexandr& Plotnikova, Olga& Skvortsov, Andrey& Skoblov, Mikhail& Reznik, Oleg& Baranova, Ancha. 2018. Reperfusion Activates AP-1 and Heat Shock Response in Donor Kidney Parenchyma after Warm Ischemia. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1127462
Modern Language Association (MLA)
Reznik, Alexandr…[et al.]. Reperfusion Activates AP-1 and Heat Shock Response in Donor Kidney Parenchyma after Warm Ischemia. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1127462
American Medical Association (AMA)
Reznik, Alexandr& Plotnikova, Olga& Skvortsov, Andrey& Skoblov, Mikhail& Reznik, Oleg& Baranova, Ancha. Reperfusion Activates AP-1 and Heat Shock Response in Donor Kidney Parenchyma after Warm Ischemia. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1127462
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127462