Expression of miR-652-3p and Effect on Apoptosis and Drug Sensitivity in Pediatric Acute Lymphoblastic Leukemia
Joint Authors
Jiang, Qian
Lu, Xiaojing
Huang, Pengli
Gao, Chao
Zhao, Xiaoxi
Xing, Tianyu
Li, Gang
Bao, Shilai
Zheng, Huyong
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
MicroRNAs (miRNAs) expression profiles were screened in plasma samples from pediatric patients with acute lymphoblastic leukemia (ALL) and healthy controls, using qRT-PCR-based TaqMan low-density miRNA arrays.
MiR-652-3p (a circulating miRNA) was downregulated in new diagnosis (ND) patients compared with healthy controls.
The levels of miR652-3p were restored in complete remission (CR) but were downregulated again in disease relapse (RE).
The expression pattern of miR-652-3p was validated in bone marrow (BM) samples from other pediatric ALL patients.
MiR-652-3p was significantly upregulated in BM when the patients (n=86) achieved CR, as compared with the matched ND samples (p<0.001).
Moreover, the miR-652-3p levels in BM decreased again in two patients at RE.
In addition, the lymphoblastic leukemia cell lines Reh and RS4:11 were found to have lower levels of miR-625-3p than the normal B-cell line.
Overexpression of miR-652-3p using agomir increased the sensitivity to vincristine and cytarabine (all p<0.05) and promoted apoptosis (both p<0.05) in Reh and RS4:11 cells.
In conclusion, the results suggested that a low level of miR-652-3p might be involved in the pathogenesis of pediatric ALL.
Overexpression of miR-652-3p might suppress lymphoblastic leukemia cells, promoting apoptosis and increasing sensitivity to chemotherapeutic drugs.
American Psychological Association (APA)
Jiang, Qian& Lu, Xiaojing& Huang, Pengli& Gao, Chao& Zhao, Xiaoxi& Xing, Tianyu…[et al.]. 2018. Expression of miR-652-3p and Effect on Apoptosis and Drug Sensitivity in Pediatric Acute Lymphoblastic Leukemia. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1127474
Modern Language Association (MLA)
Jiang, Qian…[et al.]. Expression of miR-652-3p and Effect on Apoptosis and Drug Sensitivity in Pediatric Acute Lymphoblastic Leukemia. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1127474
American Medical Association (AMA)
Jiang, Qian& Lu, Xiaojing& Huang, Pengli& Gao, Chao& Zhao, Xiaoxi& Xing, Tianyu…[et al.]. Expression of miR-652-3p and Effect on Apoptosis and Drug Sensitivity in Pediatric Acute Lymphoblastic Leukemia. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1127474
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127474