Evidence of Insecticide Resistance to Pyrethroids and Bendiocarb in Anopheles funestus from Tsararano, Marovoay District, Madagascar
Joint Authors
Rakotondranaivo, Tsiriniaina
Randriamanarivo, Solohery Fanomezana
Tanjona, Mihajarilala Rakotoniaina
Vigan-Womas, Inès
Randrianarivelojosia, Milijaona
Ndiath, Mamadou Ousmane
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Introduction.
In Madagascar, malaria control relies on the countrywide use of long lasting insecticide treated bed nets (LLINs) and on indoor residual spraying (IRS) in the central highland area as well as a small area on the eastern coast.
We tested insecticide resistance mechanisms of Anopheles funestus from Tsararano, a malaria endemic village in the coastal health district of Marovoay.
Methods.
Insecticide susceptibility bioassays were done in July 2017 on first-generation Anopheles funestus (F1) to assess (i) the susceptibility to permethrin (0.05%), deltamethrin (0.05%), DDT (4%), malathion (5%), fenitrothion (1%), and bendiocarb (0.1%); (ii) the effect of preexposure to the piperonyl butoxide (PBO) synergist; and (iii) the enzymatic activities of cytochrome P450, esterases, and glutathione S-transferases (GST).
Results.
Our results demonstrated that An.
funestus was phenotypically resistant to pyrethroids and bendiocarb, with a mortality rate (MR) of 33.6% (95%CI: 24.5-43.7%) and 86% (95%CI: 77.6-92.1%), respectively.
In contrast, An.
funestus were 100% susceptible to DDT and organophosphates (malathion and fenitrothion).
Preexposure of An.
funestus to PBO synergist significantly restored the susceptibility to bendiocarb (MR=100%) and increased the MR in the pyrethroid group, from 96% (95%CI: 90.0-98.9%) to 100% for deltamethrin and permethrin, respectively (χ2 = 43, df = 3, P< 0.0001).
Enzymatic activities of cytochrome P450 and α-esterases were significantly elevated among An.
funestus compared with the IPM reference strain (Mann-Whitney U= 30, P<0.0001; U = 145.5, P <0.0001, respectively).
No significant differences of β-esterases activities compared to the IPM reference strain were observed (Mann-Whitney U = 392.5, P = 0.08).
Conclusion.
In Tsararano, despite the absence of an IRS programme, there is evidence of high levels of insecticide resistance to pyrethroids and bendiocarb in An.
funestus.
Biochemical data indicated that a metabolic resistance mechanism through the cytochrome P450 genes is operating in the An.
funestus population.
American Psychological Association (APA)
Rakotondranaivo, Tsiriniaina& Randriamanarivo, Solohery Fanomezana& Tanjona, Mihajarilala Rakotoniaina& Vigan-Womas, Inès& Randrianarivelojosia, Milijaona& Ndiath, Mamadou Ousmane. 2018. Evidence of Insecticide Resistance to Pyrethroids and Bendiocarb in Anopheles funestus from Tsararano, Marovoay District, Madagascar. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1127521
Modern Language Association (MLA)
Rakotondranaivo, Tsiriniaina…[et al.]. Evidence of Insecticide Resistance to Pyrethroids and Bendiocarb in Anopheles funestus from Tsararano, Marovoay District, Madagascar. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1127521
American Medical Association (AMA)
Rakotondranaivo, Tsiriniaina& Randriamanarivo, Solohery Fanomezana& Tanjona, Mihajarilala Rakotoniaina& Vigan-Womas, Inès& Randrianarivelojosia, Milijaona& Ndiath, Mamadou Ousmane. Evidence of Insecticide Resistance to Pyrethroids and Bendiocarb in Anopheles funestus from Tsararano, Marovoay District, Madagascar. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1127521
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127521