Analysis of Titin in Red and White Muscles: Crucial Role on Muscle Contractions Using a Fish Model
Joint Authors
Chang, Nen-Chung
Hsu, Cheng-Chen
Wu, Ming-Ping
Chung, Chi-Li
Chiu, Wan-Chun
Chen, Hui-Min
Fong, Tsorng-Harn
Chou, Duen-Suey
Sheu, Joen-Rong
Jayakumar, Thanasekaran
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-18
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Several studies have compared molecular components between red and white skeletal muscles in mammals.
However, mammalian skeletal muscles are composed of mixed types of muscle fibers.
In the current study, we analyzed and compared the distributions of titin, lipid, phosphate ions, and fatty acid levels in red and white muscles using a fish model (Tilapia), which is rich in red and white muscles, and these are well separated.
Oil-red O staining showed that red muscle had more-abundant lipids than did white muscle.
A time-of-flight secondary-ion mass spectrometric (TOF-SIMS) analysis revealed that red muscle possessed high levels of palmitic acid and oleic acid, but white muscle contained more phosphate ions.
Moreover, elastica-van Gieson (EVG) and Mito-Tracker green FM staining showed that collagen and elastic fibers were highly, respectively, distributed in connective tissues and mitochondria in red muscle.
An electron micrographic analysis indicated that red muscle had a relatively higher number of mitochondria and longer sarcomere lengths and Z-line widths, while myofibril diameters were thicker in white muscle.
Myofibrillar proteins separated by SDS-PAGE showed that the major giant protein, titin, was highly expressed in white muscle than in red muscle.
Furthermore, ratios of titin to myosin heavy chain (MHC) (titin/MHC) were about 1.3 times higher in white muscle than red muscle.
We postulated that white muscle is fit for short and strong contractile performance due to high levels of titin and condensed sarcomeres, whereas red muscle is fit for low intensity and long-lasting activity due to high levels of lipids and mitochondria and long sarcomeres.
American Psychological Association (APA)
Wu, Ming-Ping& Chang, Nen-Chung& Chung, Chi-Li& Chiu, Wan-Chun& Hsu, Cheng-Chen& Chen, Hui-Min…[et al.]. 2018. Analysis of Titin in Red and White Muscles: Crucial Role on Muscle Contractions Using a Fish Model. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1127536
Modern Language Association (MLA)
Wu, Ming-Ping…[et al.]. Analysis of Titin in Red and White Muscles: Crucial Role on Muscle Contractions Using a Fish Model. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1127536
American Medical Association (AMA)
Wu, Ming-Ping& Chang, Nen-Chung& Chung, Chi-Li& Chiu, Wan-Chun& Hsu, Cheng-Chen& Chen, Hui-Min…[et al.]. Analysis of Titin in Red and White Muscles: Crucial Role on Muscle Contractions Using a Fish Model. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1127536
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127536