PTX3: A Potential Biomarker in Thyroid Associated Ophthalmopathy
Joint Authors
Mou, Pei
Chen, Ziyu
Jiang, Lihong
Cheng, Jinwei
Wei, Ruili
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-20
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background.
Thyroid associated ophthalmopathy (TAO) is an autoimmune disease, which involves inflammation and tissue remodeling.
Pentraxin-3 (PTX3) is a component of innate immune system and recently implicated in autoimmunity.
This observation may indicate that PTX3 participates in the inflammatory process of TAO.
Methods.
All studies were performed on TAO patients and healthy controls (45: 28 in total).
RNA-seq was used to detect differential gene expression of orbital adipose-connective tissue.
Quantitative PCR was performed to verify the results.
PTX3 protein in orbital adipose-connective tissues was visualized by immunohistochemistry (IHC).
PTX3 concentration in serum was determined by enzyme-linked immunosorbent assay (ELISA).
Results.
RNA-seq showed 1.86-log2FC higher PTX3 expression in the orbital adipose-connective tissues from TAO group than controls (FDR = 0.0059).
qPCR confirmed the difference (5.59-fold increase, p=0.0012).
The presence of PTX3 protein was demonstrated.
Orbital adipose tissue from healthy controls showed weak staining for PTX3 while tissue from TAO group was strongly positive.
Serum PTX3 concentration was significantly elevated in patients when compared to the control group (1.9-fold increase; p<0.0001).
Conclusions.
Patients with TAO showed increased presence of PTX3 in orbital tissue and serum, which may suggest a potential relationship of PTX3 and TAO.
American Psychological Association (APA)
Mou, Pei& Chen, Ziyu& Jiang, Lihong& Cheng, Jinwei& Wei, Ruili. 2018. PTX3: A Potential Biomarker in Thyroid Associated Ophthalmopathy. BioMed Research International،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1127630
Modern Language Association (MLA)
Mou, Pei…[et al.]. PTX3: A Potential Biomarker in Thyroid Associated Ophthalmopathy. BioMed Research International No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1127630
American Medical Association (AMA)
Mou, Pei& Chen, Ziyu& Jiang, Lihong& Cheng, Jinwei& Wei, Ruili. PTX3: A Potential Biomarker in Thyroid Associated Ophthalmopathy. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1127630
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127630