![](/images/graphics-bg.png)
Study on miRNAs in Pan-Cancer of the Digestive Tract Based on the Illumina HiSeq System Data Sequencing
Joint Authors
Lai, Chun-hui
Liang, Xu-zhi
Liang, Xiu-yun
Ma, Sheng-jun
Li, Jun-guo
Shi, Ming-fang
Zhu, Xu
Lan, Hui-hua
Zeng, Jiang-hui
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-24, 24 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-10-16
Country of Publication
Egypt
No. of Pages
24
Main Subjects
Abstract EN
Objective.
miRNA has gained attention as a therapeutic target in various malignancies.
The proposal of this study was to investigate the biological functions of key miRNAs and target genes in cancers of the digestive tract which include esophageal carcinoma (ESCA), gastric adenocarcinoma (GAC), colon adenocarcinoma (COAD), and rectal adenocarcinoma (READ).
Materials and Methods.
After screening differentially expressed miRNAs (DEMIs) and differentially expressed mRNAs (DEMs) in four digestive cancers from The Cancer Genome Atlas (TCGA) database, the diagnostic value of above DEMIs was evaluated by receiver-operating characteristic (ROC) curve analysis.
Then, corresponding DEMIs’ target genes were predicted by miRWalk 2.0.
Intersection of predicted target genes and DEMs was taken as the target genes of DEMIs, and miRNA-mRNA regulatory networks between DEMIs and target genes were constructed.
Meanwhile, the univariate Cox risk regression model was used to screen miRNAs with distinct prognostic value, and Kaplan–Meier analysis was used to determine their significance of prognosis.
Furthermore, we performed bioinformatics methods including protein-protein interaction (PPI) networks, gene ontology (GO) annotation, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, and gene group RIDA analysis by Gene-Cloud of Biotechnology Information (GCBI) to explore the function and molecular mechanisms of DEMIs and predicted target genes in tumor development.
Results.
Eventually, 3 DEMIs (miR-7-3, miR-328, and miR-323a) with significant prognostic value were obtained.
In addition, 3 DEMIs (miR-490-3p, miR-133a-3p, and miR-552-3p) and 281 target genes were identified, and the 3 DEMIs showed high diagnostic value in READ and moderate diagnostic value in ESCA, GAC, and COAD.
Also, the miRNA-mRNA regulatory network with 3 DEMIs and 281 overlapping genes was successfully established.
Functional enrichment analysis showed that 281 overlapping genes were mainly related to regulation of cell proliferation, cell migration, and PI3K-Akt signaling pathway.
Conclusion.
The diagnostic value and prognostic value of significant DEMIs in cancers of the digestive tract were identified, which may provide a novel direction for treatment and prognosis improvement of cancers of the digestive tract.
American Psychological Association (APA)
Lai, Chun-hui& Liang, Xu-zhi& Liang, Xiu-yun& Ma, Sheng-jun& Li, Jun-guo& Shi, Ming-fang…[et al.]. 2019. Study on miRNAs in Pan-Cancer of the Digestive Tract Based on the Illumina HiSeq System Data Sequencing. BioMed Research International،Vol. 2019, no. 2019, pp.1-24.
https://search.emarefa.net/detail/BIM-1127664
Modern Language Association (MLA)
Lai, Chun-hui…[et al.]. Study on miRNAs in Pan-Cancer of the Digestive Tract Based on the Illumina HiSeq System Data Sequencing. BioMed Research International No. 2019 (2019), pp.1-24.
https://search.emarefa.net/detail/BIM-1127664
American Medical Association (AMA)
Lai, Chun-hui& Liang, Xu-zhi& Liang, Xiu-yun& Ma, Sheng-jun& Li, Jun-guo& Shi, Ming-fang…[et al.]. Study on miRNAs in Pan-Cancer of the Digestive Tract Based on the Illumina HiSeq System Data Sequencing. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-24.
https://search.emarefa.net/detail/BIM-1127664
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127664