The Bioactive Substance Secreted by MSC Retards Mouse Aortic Vascular Smooth Muscle Cells Calcification
Joint Authors
Wang, Shuangshuang
Tong, Maoqing
Hu, Siwang
Chen, Xiaomin
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Vascular calcification, which is associated with low-level chronic inflammation, is a complication that occurs during aging, atherosclerosis, chronic kidney disease, diabetes mellitus, and hyperlipaemia.
In this study, we used conditioned media from mesenchymal stem cells (MSC-CM), a source of autologous cytokines, to test the hypothesis that MSC-CM inhibits vascular smooth muscle cell (VSMC) calcification by suppressing inflammation and apoptosis.
Methods.
VSMCs were treated with β-glycerophosphate (β-GP) to induce calcification and MSC-CM was used as a treatment.
Calcium deposition was evaluated using alizarin red and von Kossa staining after a 7-day induction period.
Intracellular calcium contents were measured via the o-cresolphthalein complexone method, and alkaline phosphatase (ALP) activity was determined using the para-nitrophenyl phosphate method.
The expressions of specific-osteogenic markers, inflammatory cytokines, and apoptosis-associated genes/proteins were examined by real-time polymerase chain reaction or western blotting.
Results.
MSC-CM inhibited β-GP-induced calcium deposition in VSMCs and decreased intracellular calcium content and ALP activity.
Additionally, MSC-CM suppressed the β-GP-induced increases in BMP2, Msx2, Runx2, and osteocalcin expression.
Additionally, MSC-CM decreased the expression of TNF-α, IL-1β, and IL-6 in VSMC.
MSC-CM also partly blocked β-GP-induced VSMC apoptosis, which was associated with an increase in the Bcl-2/Bax expression ratio and a decrease in caspase-3 expression.
Conclusion.
Our study results suggest that MSC-CM can inhibit VSMC calcification.
This suggests a potential novel clinical application for MSCs in the treatment of vascular calcification and associated diseases.
American Psychological Association (APA)
Wang, Shuangshuang& Tong, Maoqing& Hu, Siwang& Chen, Xiaomin. 2018. The Bioactive Substance Secreted by MSC Retards Mouse Aortic Vascular Smooth Muscle Cells Calcification. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1127669
Modern Language Association (MLA)
Wang, Shuangshuang…[et al.]. The Bioactive Substance Secreted by MSC Retards Mouse Aortic Vascular Smooth Muscle Cells Calcification. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1127669
American Medical Association (AMA)
Wang, Shuangshuang& Tong, Maoqing& Hu, Siwang& Chen, Xiaomin. The Bioactive Substance Secreted by MSC Retards Mouse Aortic Vascular Smooth Muscle Cells Calcification. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1127669
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127669