Selection and Evaluation of Appropriate Reference Genes for RT-qPCR Normalization of Volvariella volvacea Gene Expression under Different Conditions
Joint Authors
Wang, Ying
Xu, Jiyang
Zhao, Yucheng
Qian, Jiang
Gao, Yingnv
Wu, Yingying
Chen, Hongyu
Bian, Xiaohong
Wang, Ying
Bao, Dapeng
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-09
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Volvariella volvacea (V.
volvacea), commonly referred to as Chinese (paddy straw) mushroom, is a basidiomycete with a protein-rich volva and pileus.
Selecting appropriate reference genes is a crucial step in the normalization of quantitative real-time PCR data.
Therefore, 12 candidate reference genes were selected from the V.
volvacea transcriptome based on previous studies and then BestKeeper, geNorm, and NormFinder were used to identify reference genes stably expressed during different developmental stages and conditions.
Of the 12 candidate reference genes, SPRY domain protein (SPRYp), alpha-tubulin (TUBα), cyclophilin (CYP), L-asparaginase (L-asp), and MSF1-domain-containing protein (MSF1) were the most stably expressed under different experimental conditions, while 18S ribosomal RNA (18S), 28S ribosomal RNA (28S), and beta-actin (ACTB) were the least stably expressed.
This investigation not only revealed potential factors influencing the suitability of reference genes, but also identified optimal reference genes from a pool of candidate genes under a wide range of conditions.
American Psychological Association (APA)
Qian, Jiang& Gao, Yingnv& Wang, Ying& Wu, Yingying& Wang, Ying& Zhao, Yucheng…[et al.]. 2018. Selection and Evaluation of Appropriate Reference Genes for RT-qPCR Normalization of Volvariella volvacea Gene Expression under Different Conditions. BioMed Research International،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1127710
Modern Language Association (MLA)
Qian, Jiang…[et al.]. Selection and Evaluation of Appropriate Reference Genes for RT-qPCR Normalization of Volvariella volvacea Gene Expression under Different Conditions. BioMed Research International No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1127710
American Medical Association (AMA)
Qian, Jiang& Gao, Yingnv& Wang, Ying& Wu, Yingying& Wang, Ying& Zhao, Yucheng…[et al.]. Selection and Evaluation of Appropriate Reference Genes for RT-qPCR Normalization of Volvariella volvacea Gene Expression under Different Conditions. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1127710
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127710