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Silibinin Induced Human Glioblastoma Cell Apoptosis Concomitant with Autophagy through Simultaneous Inhibition of mTOR and YAP
Joint Authors
Ren, Juan
Bai, Zhuan-Li
Tay, Vincent
Guo, Shu-Zhong
Shu, Mao-Guo
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Silibinin, also known as silybin, is the major flavonolignan isolated from Silybum marianum.
Although previous reports demonstrated that silibinin exhibits significant tumor suppressor activities in various cancers by promoting cell apoptosis, it was also shown to trigger autophagy to counteract apoptosis induced by exogenous stresses in several types of cells.
However, there is no report to address the role of silibinin induced autophagy in human A172 and SR glioblastoma cells.
Our study showed that silibinin treatment not only inhibited the metabolic activities of glioblastoma cells but also promoted their apoptosis through the regulation of caspase 3 and PARP-1 in concentration- and time-dependent manners.
Meanwhile, silibinin induced autophagy through upregulation of microtubule-associated protein a light chain 3- (LC3-) II.
And autophagy inhibition with chloroquine, a lysosomotropic agent, significantly enhanced silibinin induced glioblastoma cell apoptosis.
Moreover, silibinin dose-dependently downregulated the phosphorylation levels of mTOR at Ser-2448, p70S6K at Thr-389, and 4E-BP1 at Thr-37/46.
Furthermore, the expression of YAP, the downstream effector of Hippo signal pathway, was also suppressed by silibinin.
These results suggested that silibinin induced glioblastoma cell apoptosis concomitant with autophagy which might be due to simultaneous inhibition of mTOR and YAP and silibinin induced autophagy exerted a protective role against cell apoptosis in both A172 and SR cells.
American Psychological Association (APA)
Bai, Zhuan-Li& Tay, Vincent& Guo, Shu-Zhong& Ren, Juan& Shu, Mao-Guo. 2018. Silibinin Induced Human Glioblastoma Cell Apoptosis Concomitant with Autophagy through Simultaneous Inhibition of mTOR and YAP. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1127740
Modern Language Association (MLA)
Bai, Zhuan-Li…[et al.]. Silibinin Induced Human Glioblastoma Cell Apoptosis Concomitant with Autophagy through Simultaneous Inhibition of mTOR and YAP. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1127740
American Medical Association (AMA)
Bai, Zhuan-Li& Tay, Vincent& Guo, Shu-Zhong& Ren, Juan& Shu, Mao-Guo. Silibinin Induced Human Glioblastoma Cell Apoptosis Concomitant with Autophagy through Simultaneous Inhibition of mTOR and YAP. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1127740
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127740