Optimizing Culture Conditions by Statistical Approach to Enhance Production of Pectinase from Bacillus sp. Y1
Joint Authors
Guo, Fenfen
Gao, Peike
Li, Guanxi
Han, Xiaolong
Zhao, Jian
Li, XueZhi
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
It was found that Bacillus sp.
Y1 could secrete alkaline pectinase with suitable enzyme system for powerful and fast degumming of ramie fiber.
In this study, the medium components and fermentation conditions were optimized by some statistical methods including mixture design, fractional factorial design, central composite design and response surface methodology, and single factor method for enhancing the alkaline pectinase production.
The optimized conditions for pectinase production were that the culture was shaken at 34°C for 60 h in 50 mL of medium containing 10.5% (w/v) carbon source (consisting of 3.8% starch, 4.2% wheat bran, and 2.5% sucrose), 0.37% (NH4)2SO4, 0.3% MgSO4, and 0.1% Tween-80, with initial pH 8.2 and inoculation amount of 1.3 mL (with the OD600 of the seed medium about 5.77).
Using the optimizing conditions, the activities of polygalacturonate lyase (PGL) and polygalacturonase (PG) in fermentation liquor were increased to 2.00-fold and 3.44-fold, respectively, and the fermentation time shortened 12 hours (from 72 h to 60 h), which showed good application potential in degumming of ramie.
American Psychological Association (APA)
Guo, Fenfen& Li, XueZhi& Zhao, Jian& Li, Guanxi& Gao, Peike& Han, Xiaolong. 2019. Optimizing Culture Conditions by Statistical Approach to Enhance Production of Pectinase from Bacillus sp. Y1. BioMed Research International،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1127745
Modern Language Association (MLA)
Guo, Fenfen…[et al.]. Optimizing Culture Conditions by Statistical Approach to Enhance Production of Pectinase from Bacillus sp. Y1. BioMed Research International No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1127745
American Medical Association (AMA)
Guo, Fenfen& Li, XueZhi& Zhao, Jian& Li, Guanxi& Gao, Peike& Han, Xiaolong. Optimizing Culture Conditions by Statistical Approach to Enhance Production of Pectinase from Bacillus sp. Y1. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1127745
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127745