Efficient Biocatalytic Synthesis of Chiral Intermediate of Pregabalin Using Immobilized Talaromyces thermophilus Lipase
Joint Authors
Zheng, Yu-Guo
Ding, Xu
Tang, Xiao-Ling
Zheng, Ren-Chao
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A mutant L206F/P207F/L259F of Talaromyces thermophilus lipase (TTL) exhibited high hydrolytic activity towards 2-carboxyethyl-3-cyano-5-methylhexanoic acid ethyl ester (CNDE) for synthesis of (S)-2-carboxyethyl-3-cyano-5-methylhexanoic acid (S-CCMA), a key chiral intermediate of pregabalin.
However, low conversion at high CNDE concentration and unreusability of the free TTL mutant restricted its industrial applications.
In this study, the TTL mutant was immobilized onto epoxy resin and its catalytic properties for kinetic resolution of CNDE were investigated.
Under the optimized conditions, the immobilized lipase exhibited an increased catalytic efficiency even at a CNDE concentration of 3 M with 49.7% conversion and 95% eep.
The conversion retained higher than 46.3% even after 10 times repeated use of the immobilized lipase in n-heptane-water biphasic system.
These results demonstrated great potential of the immobilized TTL mutant for industrial production of the chiral intermediate of pregabalin.
American Psychological Association (APA)
Ding, Xu& Tang, Xiao-Ling& Zheng, Ren-Chao& Zheng, Yu-Guo. 2018. Efficient Biocatalytic Synthesis of Chiral Intermediate of Pregabalin Using Immobilized Talaromyces thermophilus Lipase. BioMed Research International،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1127753
Modern Language Association (MLA)
Ding, Xu…[et al.]. Efficient Biocatalytic Synthesis of Chiral Intermediate of Pregabalin Using Immobilized Talaromyces thermophilus Lipase. BioMed Research International No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1127753
American Medical Association (AMA)
Ding, Xu& Tang, Xiao-Ling& Zheng, Ren-Chao& Zheng, Yu-Guo. Efficient Biocatalytic Synthesis of Chiral Intermediate of Pregabalin Using Immobilized Talaromyces thermophilus Lipase. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1127753
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127753