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The Use of Phage Display and Yeast Based Expression System for the Development of a Von Willebrand Factor Propeptide Assay: Development of a Von Willebrand Factor Propeptide Assay
Joint Authors
Meiring, S. M.
Setlai, B. D. P.
Theron, C.
Bragg, R. R.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
The diagnosis of von Willebrand disease is complex due to the heterogeneity of the disease.
About eighty percent of von Willebrand disease patients are diagnosed with a quantitative defect of von Willebrand factor (VWF) where fifty percent is due to an increased clearance of von Willebrand factor.
These patients do not respond well to the treatment of choice, Desmopressin (DDAVP) due to decreased efficacy.
The ratio between the VWF propeptide and the mature VWF antigen is used to diagnose these patients.
Commercial VWF propeptide assays are too expensive for use in developing countries.
In this study, we developed a cost-effective ELISA assay.
Methods.
We first displayed VWF propeptide on yeast.
Antibody fragments were selected against the displayed VWF propeptide by using phage display technology.
The antibodies were used to develop a cost-effective VWF propeptide assay and compared to a commercial VWF propeptide assay.
Results.
Two of these antibody fragments bound specific to the VWF propeptide and not to the yeast used for the expression of the propeptides.
These purified antibody fragments were able to detect VWF propeptide in normal plasma.
Conclusion.
Our assay performed well when compared to a commercial kit.
It also showed a higher binding affinity for VWF propeptide in plasma at especially lower plasma concentrations.
American Psychological Association (APA)
Meiring, S. M.& Setlai, B. D. P.& Theron, C.& Bragg, R. R.. 2018. The Use of Phage Display and Yeast Based Expression System for the Development of a Von Willebrand Factor Propeptide Assay: Development of a Von Willebrand Factor Propeptide Assay. BioMed Research International،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1127787
Modern Language Association (MLA)
Meiring, S. M.…[et al.]. The Use of Phage Display and Yeast Based Expression System for the Development of a Von Willebrand Factor Propeptide Assay: Development of a Von Willebrand Factor Propeptide Assay. BioMed Research International No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1127787
American Medical Association (AMA)
Meiring, S. M.& Setlai, B. D. P.& Theron, C.& Bragg, R. R.. The Use of Phage Display and Yeast Based Expression System for the Development of a Von Willebrand Factor Propeptide Assay: Development of a Von Willebrand Factor Propeptide Assay. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1127787
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127787