The Differential Expression of miRNAs and a Preliminary Study on the Mechanism of miR-194-3p in Keloids
Joint Authors
Xu, Zhishan
Guo, Bingyu
Chang, Peng
Hui, Qiang
Li, Wei
Tao, Kai
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The aim of this study was to detect abnormally expressed microRNA (miRNA) in keloids and to study their functions.
The differential expression of miRNAs in keloids and normal tissue was detected by gene microarray.
MiRNA expression was verified by real-time PCR.
A luciferase reporter gene assay, western blot, and real-time PCR were used to detect the effect of miR-194-3p on RUNX2.
An MTT assay and a transwell assay were used to detect the effect of miR-194-3p in both primary cultured fibroblasts and HKF cells.
Related proteins were analysed by western blot and real-time PCR.
The expression of miR-194-3p was lower in keloids, and MiR-194-3p was shown to target RUNX2 directly.
MiR-194-3p inhibited the proliferation and migration of fibroblasts through the inhibition of CDK4 and MMP2.
MiR-194-3p and RUNX2 may become new targets for the prevention and treatment of keloids.
American Psychological Association (APA)
Xu, Zhishan& Guo, Bingyu& Chang, Peng& Hui, Qiang& Li, Wei& Tao, Kai. 2019. The Differential Expression of miRNAs and a Preliminary Study on the Mechanism of miR-194-3p in Keloids. BioMed Research International،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1127792
Modern Language Association (MLA)
Xu, Zhishan…[et al.]. The Differential Expression of miRNAs and a Preliminary Study on the Mechanism of miR-194-3p in Keloids. BioMed Research International No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1127792
American Medical Association (AMA)
Xu, Zhishan& Guo, Bingyu& Chang, Peng& Hui, Qiang& Li, Wei& Tao, Kai. The Differential Expression of miRNAs and a Preliminary Study on the Mechanism of miR-194-3p in Keloids. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1127792
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127792