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Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil
Joint Authors
Avelar, Daniel Moreira de
Carvalho, Débora Moreira
Rabello, Ana
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Visceral leishmaniasis (VL) is considered a major public health concern in Brazil and several regions of the world.
A recent advance in the diagnosis of infectious diseases was the development of loop-mediated isothermal amplification (LAMP).
The aim of this study was to develop and evaluate a new LAMP assay for detection of K26 antigen-coding gene of L.
donovani complex.
A total of 219 blood samples of immunocompetent patients, including 114 VL cases and 105 non-VL cases, were analyzed for the diagnosis of VL in the present study.
Diagnostic accuracy was calculated against a combination of parasitological and/or serological tests as a reference standard.
The results were compared to those of kDNA Leishmania-PCR.
The detection limit for the K26-Lamp assay was 1fg L.
infantum purified DNA and 100 parasites/mL within 60 min of amplification time with visual detection for turbidity.
The assay was specific for L.
donovani complex.
Sensitivity, specificity, and accuracy were 98.2%, 98.1%, and 98.2%, respectively, for K26-LAMP and 100%, 100%, and 100%, respectively, for kDNA Leishmania-PCR.
Excellent agreement was observed between K26-LAMP and kDNA Leishmania-PCR assays (K = 0.96).
A highly sensitive and specific LAMP assay targeting K26 antigen-coding gene of L.
donovani complex was developed for diagnosis in peripheral blood samples of VL patients.
American Psychological Association (APA)
Avelar, Daniel Moreira de& Carvalho, Débora Moreira& Rabello, Ana. 2019. Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil. BioMed Research International،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1127810
Modern Language Association (MLA)
Rabello, Ana…[et al.]. Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil. BioMed Research International No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1127810
American Medical Association (AMA)
Avelar, Daniel Moreira de& Carvalho, Débora Moreira& Rabello, Ana. Development and Clinical Evaluation of Loop-Mediated Isothermal Amplification (LAMP) Assay for the Diagnosis of Human Visceral Leishmaniasis in Brazil. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1127810
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127810