Revealing the Amylase Interactome in Whole Saliva Using Proteomic Approaches
Joint Authors
Moffa, Eduardo Buozi
Crosara, Karla Tonelli Bicalho
Zuanazzi, David
Xiao, Yizhi
Siqueira, Walter Luiz
Machado, Maria Aparecida de Andrade Moreira
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-31
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Understanding proteins present in saliva and their function when isolated is not enough to describe their real role in the mouth.
Due to protein-protein interactions, structural changes may occur in macromolecules leading to functional modulation or modification.
Besides amylase’s function in carbohydrate breakdown, amylase can delay proteolytic degradation of protein partners (e.g., histatin 1) when complexed.
Due to its biochemical characteristics and high abundance in saliva, amylase probably interacts with several proteins acting as a biological carrier.
This study focused on identifying interactions between amylase and other proteins found in whole saliva (WS) using proteomic approaches.
Affinity chromatography was used, followed by gel electrophoresis methods, sodium dodecyl sulfate and native, tryptic in-solution and in-gel digestion, and mass spectrometry.
We identified 66 proteins that interact with amylase in WS.
Characterization of the identified proteins suggests that acidic (pI < 6.8) and low molecular weight (MW < 56 kDa) proteins have preference during amylase complex formation.
Most of the identified proteins present biological functions related to host protection.
A new protein-amylase network was constructed using the STRING database.
Further studies are necessary to investigate individualities of the identified amylase interactors.
These observations open avenues for more comprehensive studies on not yet fully characterized biological function of amylase.
American Psychological Association (APA)
Crosara, Karla Tonelli Bicalho& Zuanazzi, David& Moffa, Eduardo Buozi& Xiao, Yizhi& Machado, Maria Aparecida de Andrade Moreira& Siqueira, Walter Luiz. 2018. Revealing the Amylase Interactome in Whole Saliva Using Proteomic Approaches. BioMed Research International،Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1127857
Modern Language Association (MLA)
Crosara, Karla Tonelli Bicalho…[et al.]. Revealing the Amylase Interactome in Whole Saliva Using Proteomic Approaches. BioMed Research International No. 2018 (2018), pp.1-15.
https://search.emarefa.net/detail/BIM-1127857
American Medical Association (AMA)
Crosara, Karla Tonelli Bicalho& Zuanazzi, David& Moffa, Eduardo Buozi& Xiao, Yizhi& Machado, Maria Aparecida de Andrade Moreira& Siqueira, Walter Luiz. Revealing the Amylase Interactome in Whole Saliva Using Proteomic Approaches. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1127857
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127857