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Vitamin D Receptor Gene Polymorphism: An Important Predictor of Arthritis Development
Joint Authors
Sheikh, Nadeem
Mukhtar, Maryam
Suqaina, Saira Kainat
Batool, Andleeb
Fatima, Naz
Mehmood, Rabia
Nazir, Sabeen
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Vitamin D is an anti-inflammatory molecule and has a role in prevention of arthritis development.
Biologically active form 1, 25(OH)2D3 of vitamin D can only exert its action after binding its definite vitamin D receptor encoded by VDR gene.
VDR gene polymorphism leads to dysfunctioning of 1, 25(OH)2D3 ultimately disease onset.
The purpose of current study was to evaluate the effect of vitamin D level and VDR gene polymorphism on rheumatoid arthritis and osteoarthritis.
Blood samples were collected from case and control after taking written consent.
Serum was separated and vitamin D level as determined from each sample by ELISA.
DNA was extracted from each blood sample and amplified by using gene specific primers.
Genotyping was performed by Sangers sequencing and PCR-RFLP technique.
It was found that vitamin D level was not significantly different among patients and controls.
The rs10735810, rs1544410, rs7975232, and rs731236 were associated with the onset of arthritis at both allelic and genotypic level (p < 0.01).
Nucleotide change on rs10735810 site leads to change of tryptophan with arginine.
The frequencies of haplotype CGAT, CGGA, CGGT, CTAA, CTAT, TGAA, TGAT, TGGA, and TTGA were higher in patients and act as risk factors of RA onset, whereas haplotypes CGAT, CGAT, CGGT, CTGA, TGAT, TGGA, TTAA, and TTGA were associated with OA onset.
In conclusion, serum vitamin D level may be normal among arthritis patients but polymorphism on VDR gene restricts vitamin D to perform its anti-inflammatory function by altering the 1, 25(OH)2 D3 binding sites.
American Psychological Association (APA)
Mukhtar, Maryam& Sheikh, Nadeem& Suqaina, Saira Kainat& Batool, Andleeb& Fatima, Naz& Mehmood, Rabia…[et al.]. 2019. Vitamin D Receptor Gene Polymorphism: An Important Predictor of Arthritis Development. BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1127867
Modern Language Association (MLA)
Mukhtar, Maryam…[et al.]. Vitamin D Receptor Gene Polymorphism: An Important Predictor of Arthritis Development. BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1127867
American Medical Association (AMA)
Mukhtar, Maryam& Sheikh, Nadeem& Suqaina, Saira Kainat& Batool, Andleeb& Fatima, Naz& Mehmood, Rabia…[et al.]. Vitamin D Receptor Gene Polymorphism: An Important Predictor of Arthritis Development. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1127867
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127867