Network Analyses of Differentially Expressed Genes in Osteoarthritis to Identify Hub Genes
Joint Authors
Yang, Qiwei
Zhang, Guizhen
Li, Zhaoyan
Du, Zhenwu
Song, Yang
Zhong, Lei
Chen, Gaoyang
Shang, Jing
Wang, Qingyu
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-16
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Osteoarthritis (OA) is the most common degenerative disease in orthopedics.
However, the cause and underlying molecular mechanisms are not clear.
This study aims to identify the hub genes and pathways involved in the occurrence of osteoarthritis.
Methods.
The raw data of GSE89408 were downloaded from the Gene Expression Omnibus (GEO) database, and the differentially expressed genes (DEGs) were identified by R software.
The DAVID database was used for pathway and gene ontology analysis, and p<0.05 and gene count >2 were set as the cut-off point.
Moreover, protein-protein interaction (PPI) network construction was applied for exploring the hub genes in osteoarthritis.
The expression levels of the top ten hub genes in knee osteoarthritis synovial membranes and controls were detected by quantitative real-time PCR system.
Results.
A total of 229 DEGs were identified in osteoarthritis synovial membranes compared with normal synovial membranes, including 145 upregulated and 84 downregulated differentially expressed genes.
The KEGG pathway analysis results showed that up-DEGs were enriched in proteoglycans in cytokine-cytokine receptor interaction, chemokine signaling pathway, rheumatoid arthritis, and TNF signaling pathway, whereas down-DEGs were enriched in the PPAR signaling pathway and AMPK signaling pathway.
The qRT-PCR results showed that the expression levels of ADIPOQ, IL6, and CXCR1 in the synovium of osteoarthritis were significantly increased (p <0.05).
American Psychological Association (APA)
Li, Zhaoyan& Zhong, Lei& Du, Zhenwu& Chen, Gaoyang& Shang, Jing& Yang, Qiwei…[et al.]. 2019. Network Analyses of Differentially Expressed Genes in Osteoarthritis to Identify Hub Genes. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1127875
Modern Language Association (MLA)
Li, Zhaoyan…[et al.]. Network Analyses of Differentially Expressed Genes in Osteoarthritis to Identify Hub Genes. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1127875
American Medical Association (AMA)
Li, Zhaoyan& Zhong, Lei& Du, Zhenwu& Chen, Gaoyang& Shang, Jing& Yang, Qiwei…[et al.]. Network Analyses of Differentially Expressed Genes in Osteoarthritis to Identify Hub Genes. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1127875
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127875