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Effect of Formaldehyde on Human Middle Ear Epithelial Cells
Joint Authors
Lee, Jun Ho
Oh, Seung Ha
Suh, Myung-Whan
Kim, Shin Hye
Choi, Ji-Woong
Park, Moo Kyun
Song, Jae-Jun
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Formaldehyde (FA) is a familiar indoor air pollutant found in everything from cosmetics to clothing, but its impact on the middle ear is unknown.
This study investigated whether FA causes cytotoxicity, inflammation, or induction of apoptosis in human middle ear epithelial cells (HMEECs).
Cell viability was investigated using the trypan blue assay and a cell counting kit (CCK-8) in HMEECs treated with FA for 4 or 24 h.
The expression of genes encoding the inflammatory cytokine tumor necrosis factor alpha (TNF-α) and mucin (MUC5AC) was analyzed using RT-PCR.
Activation of the apoptosis pathway was determined by measuring mitochondrial membrane potential (MMP), cytochrome oxidase, caspase-9/Mch6/Apaf 3, and Caspase-Glo® 3/7 activities.
The CCK-8 assay and trypan blue assay results showed a reduction in cell viability in FA-treated HMEECs.
FA also increased the cellular expression of TNF-α and MUC5AC and reduced the activities of MMP and cytochrome oxidase.
Caspase-9 activity increased in cells stimulated for 4 h, as well as caspase-3/7 activity in cells stimulated for 24 h.
The decreased cell viability, the induction of inflammation and mucin gene expression, and the activation of the apoptosis pathway together indicate a link between environmental FA exposure and the development of otitis media.
American Psychological Association (APA)
Kim, Shin Hye& Choi, Ji-Woong& Suh, Myung-Whan& Lee, Jun Ho& Oh, Seung Ha& Song, Jae-Jun…[et al.]. 2018. Effect of Formaldehyde on Human Middle Ear Epithelial Cells. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1127892
Modern Language Association (MLA)
Kim, Shin Hye…[et al.]. Effect of Formaldehyde on Human Middle Ear Epithelial Cells. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1127892
American Medical Association (AMA)
Kim, Shin Hye& Choi, Ji-Woong& Suh, Myung-Whan& Lee, Jun Ho& Oh, Seung Ha& Song, Jae-Jun…[et al.]. Effect of Formaldehyde on Human Middle Ear Epithelial Cells. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1127892
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127892