Rapid and Visual Detection of Coxiella burnetii Using Recombinase Polymerase Amplification Combined with Lateral Flow Strips
Joint Authors
Yin, Qiong
Shao, Yinxiu
Li, Suqin
Chen, Hongxia
Shen, Wanpeng
Rao, Jixian
Li, Jiameng
Li, Xiaoling
Sun, Yu
Lin, Yu
Deng, Yi
Zeng, Wenwen
Zheng, Shulong
Liu, Suyun
Li, Yuexi
Li, Yuexi
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Coxiella burnetii, a global-distributed biological warfare agent, is the causative agent of Q fever.
Correct diagnosis of Q fever is challenging and developing a fast, simple, and reliable detection method is necessary.
In this study, recombinase polymerase amplification (RPA) assay combined with lateral flow (LF) test was developed targeting 23S rRNA gene of C.
burnetii Xinqiao strain.
Primers and probe were designed and synthesized, with one set with high amplification efficiency screened for establishment of the method.
Reaction conditions were optimized.
Sensitivity, specificity, and accuracy were evaluated.
The established RPA-LF reaction could be completed in 30 minutes by combining RPA at 37°C with LF at room temperature, with visually judged results.
The method showed good specificity without recognizing other bacteria evaluated.
It detected positive plasmid and genomic DNA at levels of 10 copies/reaction and 7 copies/reaction, respectively, levels comparable to that of real-time quantitative PCR (RT-qPCR) targeting 23S rRNA gene established previously.
Both RPA-LF and RT-qPCR were used to detect C.
burnetii-infected mouse samples and the results were fully consistent.
The method showed superior detection performance and will provide technical support against C.
burnetii in resources-limited areas.
American Psychological Association (APA)
Li, Yuexi& Yin, Qiong& Shao, Yinxiu& Li, Suqin& Chen, Hongxia& Shen, Wanpeng…[et al.]. 2018. Rapid and Visual Detection of Coxiella burnetii Using Recombinase Polymerase Amplification Combined with Lateral Flow Strips. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1127916
Modern Language Association (MLA)
Li, Yuexi…[et al.]. Rapid and Visual Detection of Coxiella burnetii Using Recombinase Polymerase Amplification Combined with Lateral Flow Strips. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1127916
American Medical Association (AMA)
Li, Yuexi& Yin, Qiong& Shao, Yinxiu& Li, Suqin& Chen, Hongxia& Shen, Wanpeng…[et al.]. Rapid and Visual Detection of Coxiella burnetii Using Recombinase Polymerase Amplification Combined with Lateral Flow Strips. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1127916
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127916