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Integrated Analysis of mRNA Expression, CpG Island Methylation, and Polymorphisms in the MITF Gene in Ducks (Anas platyrhynchos)
Joint Authors
Lin, Ruiyi
Lin, Weimin
Zhou, Shiye
Chen, Qiaohui
Pan, Jiahua
Miao, Yuanxin
Zhang, Mengwen
Huang, Zhongbin
Xiao, Tianfang
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-23
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Microphthalmia-associated transcription factor (MITF) is a key regulator for the development and function of melanocytes in skin, eye, and plumage pigmentations.
Thus, the MITF was selected as a candidate gene associated with plumage coloration in ducks.
This study analyzed the mRNA expression, promoter methylation, and polymorphisms in the MITF gene in ducks with different plumage colors (Putian Black, Putian White, Liancheng White, and Longsheng Jade-green).
No expression of the MITF melanin-specific isoform (MITF-M) was detected in white feather bulbs.
By contrast, the mRNA expression levels of MITF-M were high in black feather bulbs.
Bioinformatics analysis showed that two CpG islands were present in the promoter region of the MITF gene.
The methylation level of the second CpG island was significantly lower in black feather bulbs than in white feather bulbs.
However, the methylation level of the first CpG island was not different among the feather bulbs with various colors except Liancheng White feather bulbs.
The methylation status of the whole CpG island significantly and negatively correlated with the mRNA expression of MITF-M (P<0.05).
Furthermore, four novel SNPs (single nucleotide polymorphisms) were identified in the 5′UTR, exon 4, intron 7, and intron 8 of the MITF gene.
Allele T in g.39807T>G and allele G in g.40862G>A were the predominant alleles only found in Putian White, whereas the variant A allele in g.32813G>A exhibited a high allele frequency in Liancheng White.
Collectively, these results contributed to the understanding of the function of the MITF gene in duck plumage coloration.
American Psychological Association (APA)
Lin, Ruiyi& Lin, Weimin& Zhou, Shiye& Chen, Qiaohui& Pan, Jiahua& Miao, Yuanxin…[et al.]. 2019. Integrated Analysis of mRNA Expression, CpG Island Methylation, and Polymorphisms in the MITF Gene in Ducks (Anas platyrhynchos). BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1127935
Modern Language Association (MLA)
Lin, Ruiyi…[et al.]. Integrated Analysis of mRNA Expression, CpG Island Methylation, and Polymorphisms in the MITF Gene in Ducks (Anas platyrhynchos). BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1127935
American Medical Association (AMA)
Lin, Ruiyi& Lin, Weimin& Zhou, Shiye& Chen, Qiaohui& Pan, Jiahua& Miao, Yuanxin…[et al.]. Integrated Analysis of mRNA Expression, CpG Island Methylation, and Polymorphisms in the MITF Gene in Ducks (Anas platyrhynchos). BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1127935
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127935