Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3KAKTeNOS Signaling Pathway
Joint Authors
Chen, Keyan
Nan, Wang
Zhonghang, Xu
Tongtong, Liu
Wanshu, Guo
Zhongxin, Xu
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-25
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background/Aims.
Epigallocatechin-3-gallate (EGCG) has neuroprotective effects and the ability to resist amyloidosis.
This study observed the protective effect of EGCG against neuronal injury in rat models of middle cerebral artery occlusion (MCAO) and investigated the mechanism of action of PI3K/AKT/eNOS signaling pathway.
Methods.
Rat models of permanent MCAO were established using the suture method.
Rat behavior was measured using neurological deficit score.
Pathology and apoptosis were measured using HE staining and TUNEL.
Oxidative stress and brain injury markers were examined using ELISA.
Apoptosis-related proteins and PI3K/AKT/eNOS signaling pathway were determined using western blot assay and immunohistochemistry.
Results.
EGCG decreased neurological function score, protected nerve cells, inhibited neuronal apoptosis, and inhibited oxidative stress injury and brain injury markers level after MCAO.
EGCG reduced the apoptotic rate of neurons, increased the expression of Bcl-2, and decreased the expression of Caspase-3 and Bax.
After LY294002 suppressed the PI3K pathway, the protective effect of EGCG decreased after administration of PI3K inhibitors.
Conclusion.
EGCG has a protective effect on rat brain injury induced by MCAO, possibly by modulating the PI3K/AKT/eNOS signaling pathway.
American Psychological Association (APA)
Nan, Wang& Zhonghang, Xu& Chen, Keyan& Tongtong, Liu& Wanshu, Guo& Zhongxin, Xu. 2018. Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3KAKTeNOS Signaling Pathway. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1127950
Modern Language Association (MLA)
Nan, Wang…[et al.]. Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3KAKTeNOS Signaling Pathway. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1127950
American Medical Association (AMA)
Nan, Wang& Zhonghang, Xu& Chen, Keyan& Tongtong, Liu& Wanshu, Guo& Zhongxin, Xu. Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3KAKTeNOS Signaling Pathway. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1127950
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127950