Influence of Hypoxic Preservation Temperature on Endothelial Cells and Kidney Integrity
Joint Authors
Hauet, T.
Goujon, Jean Michel
Steichen, Clara
Couturier, Pierre
Tillet, Solenne
Mallet, Vanessa
Coudroy, Rémi
Hannaert, Patrick
Giraud, S.
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-04
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Ischemia-reperfusion (IR) injury is unavoidable during organ transplantation and impacts graft quality.
New paradigms are emerging including preservation at higher temperature than “hypothermia” or “cold”: although 4°C remains largely used for kidney preservation, recent studies challenged this choice.
We and others hypothesized that a higher preservation temperature, closer to physiological regimen, could improve organ quality.
For this purpose, we used an in vitro model of endothelial cells exposed to hypoxia-reoxygenation sequence (mimicking IR) and an ex vivo ischemic pig kidneys static storage model.
In vitro, 19°C, 27°C, and 32°C provided protection against injuries versus 4°C, by reducing cell death, mitochondrial dysfunction, leukocyte adhesion, and inflammation.
However, ex vivo, the benefits of 19°C or 32°C were limited, showing similar levels of tissue preservation damage.
Ex vivo 4°C-preserved kidneys displayed a trend towards reduced damage, including apoptosis.
Macrophage infiltration, tubulitis, and necrosis were increased in the 19°C and 32°C versus 4°C preserved kidneys.
Thus, despite a trend for an advantage of subnormothermia as preservation temperature, our in vitro and ex vivo models bring different insights in terms of preservation temperature effect.
This study suggests that temperature optimization for kidney preservation will require thorough investigation, combining the use of complementary relevant models and the design of elaborated preservation solution and new technologies.
American Psychological Association (APA)
Giraud, S.& Steichen, Clara& Couturier, Pierre& Tillet, Solenne& Mallet, Vanessa& Coudroy, Rémi…[et al.]. 2019. Influence of Hypoxic Preservation Temperature on Endothelial Cells and Kidney Integrity. BioMed Research International،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1127985
Modern Language Association (MLA)
Giraud, S.…[et al.]. Influence of Hypoxic Preservation Temperature on Endothelial Cells and Kidney Integrity. BioMed Research International No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1127985
American Medical Association (AMA)
Giraud, S.& Steichen, Clara& Couturier, Pierre& Tillet, Solenne& Mallet, Vanessa& Coudroy, Rémi…[et al.]. Influence of Hypoxic Preservation Temperature on Endothelial Cells and Kidney Integrity. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1127985
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1127985