Human MLL-AF9 Overexpression Induces Aberrant Hematopoietic Expansion in Zebrafish
Joint Authors
Zhou, Jianfeng
Tan, Jiaqi
Zhao, Lei
Wang, Gaoxiang
Li, Tongjuan
Li, Dan
Xu, Qian
Chen, Xing
Shang, Zhen
Wang, Jue
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The 11q23 of the mixed lineage leukemia 1 (MLL1) gene plays a crucial role in early embryonic development and hematopoiesis.
The MLL-AF9 fusion gene, resulting from chromosomal translocation, often leads to acute myeloid leukemia with poor prognosis.
Here, we generated a zebrafish model expressing the human MLL-AF9 fusion gene.
Microinjection of human MLL-AF9 mRNA into zebrafish embryos resulted in enhanced hematopoiesis and the activation of downstream genes such as meis1 and hox cluster genes.
Embryonic MLL-AF9 expression upregulated HSPC and myeloid lineage markers.
Doxorubicin and MI-2 (a menin inhibitor) treatments significantly restored normal hematopoiesis in MLL-AF9-expressing animals.
This study provides insight into the role of MLL-AF9 in zebrafish hematopoiesis and establishes a robust and efficient in vivo model for high-throughput drug screening.
American Psychological Association (APA)
Tan, Jiaqi& Zhao, Lei& Wang, Gaoxiang& Li, Tongjuan& Li, Dan& Xu, Qian…[et al.]. 2018. Human MLL-AF9 Overexpression Induces Aberrant Hematopoietic Expansion in Zebrafish. BioMed Research International،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1128020
Modern Language Association (MLA)
Tan, Jiaqi…[et al.]. Human MLL-AF9 Overexpression Induces Aberrant Hematopoietic Expansion in Zebrafish. BioMed Research International No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1128020
American Medical Association (AMA)
Tan, Jiaqi& Zhao, Lei& Wang, Gaoxiang& Li, Tongjuan& Li, Dan& Xu, Qian…[et al.]. Human MLL-AF9 Overexpression Induces Aberrant Hematopoietic Expansion in Zebrafish. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1128020
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128020