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Usnea Acid as Multidrug Resistance (MDR) Reversing Agent against Human Chronic Myelogenous Leukemia K562ADR Cells via an ROS Dependent Apoptosis
Joint Authors
Wang, Wenjing
Qiao, Luxin
Niu, Shubin
Wei, Feili
Yin, Jiming
Wang, Shanshan
Ouyang, Yabo
Chen, Dexi
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Purpose.
Multidrug resistance (MDR) is a major obstacle in chemotherapy of leukemia treatments.
In this paper, we investigated Usnea Acid (UA) as MDR reversal agent on hematologic K562/ADR cells via ROS dependent apoptosis.
Methods.
CCK8 assay was used to measure cell viability rate of K562/ADR.
Intracellular reactive oxygen species (ROS) generation, cell cycle distribution, cell apoptosis were measured with flow cytometry, respectively.
Proteins related to apoptosis were measured by Western blot.
Intracellular Adriamycin accumulation was observed by confocal microscopy and measured by flow cytometry.
Results.
In vitro study showed intracellular Adriamycin accumulation was remarkably increased by UA.
Cell viability treated with Adr (4 μM) was decreased from 89.8% ± 4.7 to 32% ± 8.9 by combined with UA (4 μM).
Adr-induced apoptosis and G1/G0 phase cell cycle arrest were remarkably increased by UA, as well as, intracellular ROS level.
However, MDR reversing activity of UA was inhibited by N-acetyl cysteine (NAC), a ROS scavenger.
Conclusion.
These data provide compelling evidence that UA is a promising agent against MDR in leukemia cell line and suggest a promising therapeutic approach for leukemia.
American Psychological Association (APA)
Wang, Wenjing& Niu, Shubin& Qiao, Luxin& Wei, Feili& Yin, Jiming& Wang, Shanshan…[et al.]. 2019. Usnea Acid as Multidrug Resistance (MDR) Reversing Agent against Human Chronic Myelogenous Leukemia K562ADR Cells via an ROS Dependent Apoptosis. BioMed Research International،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1128090
Modern Language Association (MLA)
Wang, Wenjing…[et al.]. Usnea Acid as Multidrug Resistance (MDR) Reversing Agent against Human Chronic Myelogenous Leukemia K562ADR Cells via an ROS Dependent Apoptosis. BioMed Research International No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1128090
American Medical Association (AMA)
Wang, Wenjing& Niu, Shubin& Qiao, Luxin& Wei, Feili& Yin, Jiming& Wang, Shanshan…[et al.]. Usnea Acid as Multidrug Resistance (MDR) Reversing Agent against Human Chronic Myelogenous Leukemia K562ADR Cells via an ROS Dependent Apoptosis. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1128090
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128090