![](/images/graphics-bg.png)
Aminophylline and Ephedrine, but Not Flumazenil, Inhibit the Activity of the Excitatory Amino Acid Transporter 3 Expressed in Xenopus Oocytes and Reverse the Increased Activity by Propofol
Joint Authors
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
We investigated the effects of flumazenil, aminophylline, and ephedrine on the excitatory amino acid transporter type 3 (EAAT3) activity and the interaction with propofol.
EAAT3 was expressed in the Xenopus oocytes.
L-Glutamate-induced membrane currents were measured using the two-electrode voltage clamp at various drug concentrations.
Oocytes were preincubated with protein kinase C- (PKC-) activator, or inhibitor, and phosphatidylinositol 3-kinase (PI3K) inhibitor.
To study the interaction with propofol, oocytes were exposed to propofol, propofol + aminophylline, or ephedrine.
Aminophylline and ephedrine significantly decreased EAAT3 activity.
Aminophylline (95 μM) and ephedrine (1.19 μM) significantly decreased Vmax, but not Km of EAAT3, for glutamate.
The phorbol 12-myristate-13-acetate-induced increase in EAAT3 activity was abolished by aminophylline or ephedrine.
The decreased EAAT3 activities by PKC inhibitors (staurosporine, chelerythrine) and PI3K inhibitor (wortmannin) were not significantly different from those by aminophylline or ephedrine, as well as those by PKC inhibitors or PI3K inhibitor + aminophylline or ephedrine.
The enhanced EAAT3 activities induced by propofol were significantly abolished by aminophylline or ephedrine.
Aminophylline and ephedrine inhibit EAAT3 activity via PKC and PI3K pathways and abolish the increased EAAT3 activity by propofol.
Our results indicate a novel site of action for aminophylline and ephedrine.
American Psychological Association (APA)
Moon, Sohyeon& Baik, Hee Jung. 2018. Aminophylline and Ephedrine, but Not Flumazenil, Inhibit the Activity of the Excitatory Amino Acid Transporter 3 Expressed in Xenopus Oocytes and Reverse the Increased Activity by Propofol. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1128111
Modern Language Association (MLA)
Moon, Sohyeon& Baik, Hee Jung. Aminophylline and Ephedrine, but Not Flumazenil, Inhibit the Activity of the Excitatory Amino Acid Transporter 3 Expressed in Xenopus Oocytes and Reverse the Increased Activity by Propofol. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1128111
American Medical Association (AMA)
Moon, Sohyeon& Baik, Hee Jung. Aminophylline and Ephedrine, but Not Flumazenil, Inhibit the Activity of the Excitatory Amino Acid Transporter 3 Expressed in Xenopus Oocytes and Reverse the Increased Activity by Propofol. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1128111
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128111