Elevated Platelet Galectin-3 and Rho-Associated Protein Kinase Activity Are Associated with Hemodialysis Arteriovenous Shunt Dysfunction among Subjects with Diabetes Mellitus
Joint Authors
Chen, Po-Wei
Hsu, Ling-Wei
Chang, Hsien-Yuan
Huang, Ting-Chun
Yu, Jia-Rong
Liao, Hsin-Yu
Lee, Cheng-Han
Liu, Ping-Yen
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-08
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Introduction.
Hyperglycemia is a major factor in influencing the patency rate of arteriovenous shunts, potentially associated with the RhoA/Rho-associated protein kinase (ROCK) pathway.
Besides, galectin-3 mediates thrombotic mechanisms in venous thrombosis and peripheral artery disease.
We hypothesized that high ROCK activity and galectin-3 levels are associated with arteriovenous shunt dysfunction.
Methods.
We prospectively enrolled 38 patients diagnosed with arteriovenous shunt dysfunction.
29 patients received a complete follow-up and each provided two blood samples, which were collected at the first visit for occluded status of arteriovenous shunts and 1 month later for patent status.
A Western blot assay for a myosin phosphatase target subunit (MYPT) was performed to examine Rho-kinase activity.
A Western blot assay for platelet galectin-3 and enzyme-linked immunosorbent assay (ELISA) for circulating galectin-3 were completed.
Results.
Higher platelet MYPT ratios and galectin-3 levels were identified at occluded arteriovenous shunts (MYPT ratio: 0.5 [0.3–1.4] vs.
0.4 [0.3–0.6], p = 0.01; galectin-3: 1.2 [0.4–1.6] vs.
0.7 [0.1–1.2], p = 0.0004).
The plasma galectin-3 binding protein ELISA was also higher at occluded arteriovenous shunts (8.4 [6.0–9.7] μg/mL vs.
7.1 [4.5–9.1] μg/mL, p = 0.009).
Biomarker ratios (occluded/patent status) trended high in patients with poorly controlled diabetes (MYPT ratio: 1.7 [1.0–3.0] vs.
1.1 [0.7–1.3], p = 0.06; galectin-3: 1.6 [1.3–3.4] vs.
1.1 [0.8–1.9], p = 0.05).
Conclusion.
High platelet ROCK activity and galectin-3 levels are associated with increased risk in arteriovenous shunt dysfunction, especially in patients with poorly controlled diabetes.
American Psychological Association (APA)
Chen, Po-Wei& Hsu, Ling-Wei& Chang, Hsien-Yuan& Huang, Ting-Chun& Yu, Jia-Rong& Liao, Hsin-Yu…[et al.]. 2019. Elevated Platelet Galectin-3 and Rho-Associated Protein Kinase Activity Are Associated with Hemodialysis Arteriovenous Shunt Dysfunction among Subjects with Diabetes Mellitus. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1128185
Modern Language Association (MLA)
Chen, Po-Wei…[et al.]. Elevated Platelet Galectin-3 and Rho-Associated Protein Kinase Activity Are Associated with Hemodialysis Arteriovenous Shunt Dysfunction among Subjects with Diabetes Mellitus. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1128185
American Medical Association (AMA)
Chen, Po-Wei& Hsu, Ling-Wei& Chang, Hsien-Yuan& Huang, Ting-Chun& Yu, Jia-Rong& Liao, Hsin-Yu…[et al.]. Elevated Platelet Galectin-3 and Rho-Associated Protein Kinase Activity Are Associated with Hemodialysis Arteriovenous Shunt Dysfunction among Subjects with Diabetes Mellitus. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1128185
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128185