CRISPRCas9 Knockout of Bak Mediates Bax Translocation to Mitochondria in response to TNFαCHX-induced Apoptosis
Joint Authors
Fu, Xueqi
Zhang, Jingtian
Niu, Han
Zhao, Zhizhuang Joe
Wang, Yuxiang
Zhang, Xu
Zhang, Fuqiang
Zeng, Linlin
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-09-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
TNFα/CHX-induced apoptosis is dependent on caspase-8 activation and regulated by Bcl-2.
However, the specific participants and precise mechanisms underlying this apoptotic pathway are poorly understood.
The proapoptotic proteins Bak and Bax—members of the Bcl-2 family—are essential for the functioning of the mitochondrial apoptotic pathway.
In this study, we used the CRISPR/Cas9 system to knockout Bak in the human SH-SY5Y cell line and determined the effects of this knockout on TNFα/CHX-induced apoptosis.
Our data showed that overexpression of Bcl-2 dramatically prevented TNFα/CHX-induced apoptosis, and then pro-apoptotic protein Bak was downregulated and became more resistant to TNFα/CHX-induced apoptosis, because both TNFα/CHX-induced PARP cleavage and caspase activation were blocked in BAK−/− cells or using specific siRNA, whereas Bax was dispensable in TNFα/CHX-induced apoptosis, as evidenced using specific siRNA.
Bax translocated from the cytosol into the mitochondria in response to TNFα/CHX, and CRISPR/Cas9 knockout of Bak significantly decreased this translocation.
These results indicate that TNFα/CHX-induced apoptosis does not occur in Bak−/− cells, suggesting that TNFα/CHX-induced apoptosis is Bak-dependent but Bax-independent.
American Psychological Association (APA)
Zhang, Jingtian& Niu, Han& Zhao, Zhizhuang Joe& Fu, Xueqi& Wang, Yuxiang& Zhang, Xu…[et al.]. 2019. CRISPRCas9 Knockout of Bak Mediates Bax Translocation to Mitochondria in response to TNFαCHX-induced Apoptosis. BioMed Research International،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1128296
Modern Language Association (MLA)
Zhang, Jingtian…[et al.]. CRISPRCas9 Knockout of Bak Mediates Bax Translocation to Mitochondria in response to TNFαCHX-induced Apoptosis. BioMed Research International No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1128296
American Medical Association (AMA)
Zhang, Jingtian& Niu, Han& Zhao, Zhizhuang Joe& Fu, Xueqi& Wang, Yuxiang& Zhang, Xu…[et al.]. CRISPRCas9 Knockout of Bak Mediates Bax Translocation to Mitochondria in response to TNFαCHX-induced Apoptosis. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1128296
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128296