Significant Role of Dicer and miR-223 in Adipose Tissue of Polycystic Ovary Syndrome Patients
Joint Authors
Xu, Wenming
Zuo, Tao
Qin, Lang
Chen, Jiao
Tang, Li
Chen, Hanxiao
Gao, Rui
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Polycystic ovary syndrome (PCOS) is a chronic metabolic disease that is associated with obesity and adipose tissue dysfunction.
This study aimed to explore the roles of Dicer (an enzyme that processes primary microRNAs) and microRNAs in PCOS.
Protein levels were detected by western blotting, and mRNA and microRNA levels were detected by RT-PCR.
Dicer-deficient pre-adipocytes were established by lentiviral transfection, and an miR-223 mimic and miR-223 inhibitor were used to overexpress and inhibit miR-223, respectively.
3T3-L1 cells were induced to differentiate into mature adipocytes by IBMX, insulin, and dexamethasone.
The degree of differentiation was determined by oil red O staining.
An insulin resistance model was established by exposing mature adipocytes to excessive glucose and insulin.
The protein levels of Dicer and Ago2 in adipose tissues of PCOS patients were significantly lower than those in control females.
A Dicer-deficient 3T3-L1 cell model was successfully established, whose proliferation was inhibited significantly.
Insulin-resistant mature adipocytes expressed significantly less Dicer protein than control cells.
The differentiation of Dicer-deficient 3T3-L1 cells and their expression of miR-223 and marker genes associated with adipose differentiation were reduced significantly.
Furthermore, 3T3-L1 cells showed a weaker ability to develop into mature adipocytes when miR-223 expression was inhibited.
An miR-223 mimic was used to recover the differentiation block induced by Dicer deficiency.
This rescued the expression of genes associated with adipose differentiation, although the differentiation block was not efficiently rescued.
It is concluded that insulin resistance may contribute to the decreased levels of Dicer protein in adipose tissue of PCOS patients.
This suggests that dysfunction of Dicer plays a significant role in obesity of PCOS patients.
miR-223 is a key factor in Dicer-regulated adipose differentiation, and other microRNAs may be involved in the process.
American Psychological Association (APA)
Qin, Lang& Chen, Jiao& Tang, Li& Zuo, Tao& Chen, Hanxiao& Gao, Rui…[et al.]. 2019. Significant Role of Dicer and miR-223 in Adipose Tissue of Polycystic Ovary Syndrome Patients. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1128370
Modern Language Association (MLA)
Qin, Lang…[et al.]. Significant Role of Dicer and miR-223 in Adipose Tissue of Polycystic Ovary Syndrome Patients. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1128370
American Medical Association (AMA)
Qin, Lang& Chen, Jiao& Tang, Li& Zuo, Tao& Chen, Hanxiao& Gao, Rui…[et al.]. Significant Role of Dicer and miR-223 in Adipose Tissue of Polycystic Ovary Syndrome Patients. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1128370
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128370