Oroxylin A Suppresses the Cell Proliferation, Migration, and EMT via NF-κB Signaling Pathway in Human Breast Cancer Cells

Abstract EN

Oroxylin A is a natural extract and has been reported to have a remarkable anticancer function.

However, the mechanism of its anticancer activity remains not quite clear.

In this study, we examined the inhibiting effects of Oroxylin A on breast cancer cell proliferation, migration, and epithelial-mesenchymal transition (EMT) and its possible molecular mechanism.

The cytoactive and inflammatory factors were analyzed via Cell Counting Kit-8 assay and ELISA assay, respectively.

Flow cytometry and western blotting were used to assess the cell proliferation.

In addition, a wound healing assay and transwell assay were used to detect cell invasion and migration.

qRT-PCR and western blot were employed to determine the effect of Oroxylin A on the EMT formation.

Moreover, expression level of protein related to NF-κB signaling pathway was determined by western blot.

The results revealed that Oroxylin A attenuated the cytoactivity of MDA-MB-231 cells in a dose- and a time-dependent manner.

Moreover, cell proliferation, invasion, and migration of breast cancer cells were inhibited by Oroxylin A compared to the control.

The mRNA and protein expression levels of E-cadherin were remarkably increased while N-cadherin and Vimentin remarkably decreased.

Besides, Oroxylin A suppressed the expression of inflammatory factors and NF-κB activation.

Furthermore, we also found that supplement of TNF-α reversed the effects of Oroxylin A on the cell proliferation, invasion, migration, and EMT in breast cancer cells.

Taken together, our results suggested that Oroxylin A inhibited the cell proliferation, invasion, migration, and EMT through inactivating NF-κB signaling pathway in human breast cancer cells.

These findings strongly suggest that Oroxylin A could be a therapeutic potential candidate for the treatment of breast cancer.