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Increased COUP-TFII Expression Mediates the Differentiation Imbalance of Bone Marrow-Derived Mesenchymal Stem Cells in Femoral Head Osteonecrosis
Joint Authors
Wang, Sheng-Hao
Gou, Guo-Hau
Wu, Chia-Chun
Shen, Hsain-Chung
Lin, Leou-Chyr
Pan, Ru-Yu
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-08
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Objective.
Bone marrow-derived mesenchymal stem cells (BMSCs) have multilineage differentiation potential, which allows them to progress to osteogenesis, adipogenesis, and chondrogenesis.
An imbalance of differentiation between osteogenesis and adipogenesis will result in pathologic conditions inside the bone.
This type of imbalance is also one of the pathological findings in osteonecrosis of the femoral head (ONFH).
Chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) was previously reported to mediate the differentiation of mesenchymal stem cells.
This study investigated the expression of the osteogenesis regulator Runx2, osteocalcin, the adipogenesis regulator PPARγ, and COUP-TFII in the femoral head tissue harvested from ONFH patients, and characterized the effect of COUP-TFII on the differentiation of primary BMSCs.
Methods.
Thirty patients with ONFH were recruited and separated into 3 groups: the trauma-, steroid- and alcohol-induced ONFH groups (10 patients each).
Bone specimens were harvested from patients who underwent hip arthroplasty, and another 10 specimens were harvested from femoral neck fracture patients as the control group.
Expression of the osteogenesis regulator Runx2, osteocalcin, the adipogenesis regulator PPARγ, C/EBP-α, and COUP-TFII was analyzed by Western blotting.
Primary bone marrow mesenchymal cells were harvested from ONFH cells treated with COUP-TFII RNA interference to evaluate the effect of COUP-TFII on MSCs.
Results.
ONFH patients had significantly increased expression of the adipogenesis regulator PPARγ and C/EBP-α and decreased expression of the osteogenesis regulator osteocalcin.
ONFH bone tissue also revealed higher COUP-TFII expression.
Immunohistochemical staining displayed strong COUP-TFII immunoreactivity adjacent to osteonecrotic trabecular bone.
Increased COUP-TFII expression in the bone tissue correlated with increased PPARγ and decreased osteocalcin expression.
Knockdown of COUP-TFII with siRNA in BMSCs reduced adipogenesis and increased osteogenesis in mesenchymal cells.
Conclusion.
Increased COUP-TFII expression mediates the imbalance of BMSC differentiation and progression to ONFH in patients.
This study might reveal a new target in the treatment of ONFH.
American Psychological Association (APA)
Wang, Sheng-Hao& Gou, Guo-Hau& Wu, Chia-Chun& Shen, Hsain-Chung& Lin, Leou-Chyr& Pan, Ru-Yu. 2019. Increased COUP-TFII Expression Mediates the Differentiation Imbalance of Bone Marrow-Derived Mesenchymal Stem Cells in Femoral Head Osteonecrosis. BioMed Research International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1128410
Modern Language Association (MLA)
Wang, Sheng-Hao…[et al.]. Increased COUP-TFII Expression Mediates the Differentiation Imbalance of Bone Marrow-Derived Mesenchymal Stem Cells in Femoral Head Osteonecrosis. BioMed Research International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1128410
American Medical Association (AMA)
Wang, Sheng-Hao& Gou, Guo-Hau& Wu, Chia-Chun& Shen, Hsain-Chung& Lin, Leou-Chyr& Pan, Ru-Yu. Increased COUP-TFII Expression Mediates the Differentiation Imbalance of Bone Marrow-Derived Mesenchymal Stem Cells in Femoral Head Osteonecrosis. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1128410
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128410