Gastrodin Ameliorates Acute Rejection via IRE1αTRAF2NF-κB in Rats Receiving Liver Allografts
Joint Authors
Wu, Hao
Duan, Shigang
Yuan, Fangchao
Xu, Xuesong
Wu, Yakun
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Liver transplantation (LT) is currently an effective treatment for end-stage liver disease, but the occurrence of acute rejection (AR) is still the main problem to be solved.
The present study aimed to evaluate the effect of gastrodin (GAS) on LT.
Methods.
Rat transplant models were established and divided into SHAM, LT, GAS-L (50 mg/kg GAS), and GAS-H (100 mg/kg GAS) groups.
The liver function, inflammatory factors, liver histopathology, survival of rats, number of M2-type macrophages, liver cell apoptosis, and pathway proteins were assayed at 7 days and 14 days after the operations.
Results.
With increasing GAS concentrations, liver function, expression of proinflammatory factors in the liver, and expression of M2-type molecules in macrophages were significantly improved, and the survival time of rats was significantly prolonged (P<0.05).
All rats treated with low or high doses of GAS were judged to have nondeterministic acute rejection.
Flow cytometry showed that liver cell apoptosis was decreased significantly in the GAS-L and GAS-H groups after GAS administration compared with apoptosis and differentiation in the LT group (P<0.05).
Expression levels of Caspase-3, Bad, and Bax proteins were decreased, and the expression of the antiapoptotic protein Bcl-2 was increased in the GAS-L and GAS-H groups (P<0.05).
Mechanistically, the ERS-related IRE1α/TRAF2/NF-κB pathway was suppressed by GAS, and GAS acted mainly on intrahepatic macrophages to affect AR and reduce ROS production (P<0.05).
Conclusion.
GAS ameliorated AR by inhibiting the IRE1α/TRAF2/NF-κB pathway in LT.
American Psychological Association (APA)
Yuan, Fangchao& Xu, Xuesong& Wu, Yakun& Duan, Shigang& Wu, Hao. 2019. Gastrodin Ameliorates Acute Rejection via IRE1αTRAF2NF-κB in Rats Receiving Liver Allografts. BioMed Research International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1128426
Modern Language Association (MLA)
Yuan, Fangchao…[et al.]. Gastrodin Ameliorates Acute Rejection via IRE1αTRAF2NF-κB in Rats Receiving Liver Allografts. BioMed Research International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1128426
American Medical Association (AMA)
Yuan, Fangchao& Xu, Xuesong& Wu, Yakun& Duan, Shigang& Wu, Hao. Gastrodin Ameliorates Acute Rejection via IRE1αTRAF2NF-κB in Rats Receiving Liver Allografts. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1128426
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128426