Discovery and Validation of Serum MicroRNAs as Early Diagnostic Biomarkers for Prostate Cancer in Chinese Population
Joint Authors
Xu, Huan
Lyu, Ji
Zhao, Lin
Wang, Fubo
Ji, Jin
Cao, Zhi
Shi, Xiaolei
Zhu, Yasheng
Zhang, Chao
Guo, Fei
Yang, Bo
Sun, Yinghao
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-08-25
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Prostate cancer (PCa) incidence has been rising in Chinese population.
Current PSA-based biopsy has limited positive rate.
Our research focused on development of serum markers for the diagnosis of PCa in patients with elevated PSA.
miRNAs are found to be aberrantly expressed in many types of cancer.
They are readily detectable in plasma and serum.
Currently, miRNAs are being evaluated as potential prognostic and diagnostic tools for many types of cancer.
We first profiled global serum miRNAs in a pilot set of PCa and benign prostatic hyperplasia (BPH) cases undergoing TRUS-guided prostate biopsy due to elevated PSA levels.
A total of 20 differentially expressed miRNAs were discovered by high throughput microarray for further testing using qRT-PCR.
In the training phase with 78 PCa and 77 BPH cases, miR-365a-3p, miR-4286, miR-424-5p, miR-27a-3p, and miR-29b-3p were found to have potential diagnostic value.
The Logistics regression equation was established by 5 parameters including PSA, prostate volume, miR-4286, miR-27a-3p, and miR-29b-3p and ROC analysis of this model was made with AUC up to 0.892 (95% CI: 0.832-0.937, sensitivity 78.95%, and specificity 92.21%).
The panel had excellent diagnostic performance and its significance was confirmed in 100 serum samples in the validation cohort.
Overall, we found a panel of serum microRNAs that have considerable clinical significance in detecting early-stage prostate cancer.
When combined with PSA and prostate volume, these microRNAs exhibit favorable diagnostic potency.
American Psychological Association (APA)
Lyu, Ji& Zhao, Lin& Wang, Fubo& Ji, Jin& Cao, Zhi& Xu, Huan…[et al.]. 2019. Discovery and Validation of Serum MicroRNAs as Early Diagnostic Biomarkers for Prostate Cancer in Chinese Population. BioMed Research International،Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1128457
Modern Language Association (MLA)
Lyu, Ji…[et al.]. Discovery and Validation of Serum MicroRNAs as Early Diagnostic Biomarkers for Prostate Cancer in Chinese Population. BioMed Research International No. 2019 (2019), pp.1-9.
https://search.emarefa.net/detail/BIM-1128457
American Medical Association (AMA)
Lyu, Ji& Zhao, Lin& Wang, Fubo& Ji, Jin& Cao, Zhi& Xu, Huan…[et al.]. Discovery and Validation of Serum MicroRNAs as Early Diagnostic Biomarkers for Prostate Cancer in Chinese Population. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-9.
https://search.emarefa.net/detail/BIM-1128457
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128457