Studies on the Interaction between Three Small Flavonoid Molecules and Bovine Lactoferrin
Joint Authors
Niu, Bing
Liu, Zhenmin
Huang, Junyi
Ma, Qiaorong
He, Ziyu
Niu, Zhidian
Zhang, Mengying
Pan, Liu
Qu, Xiaosheng
Yu, Jun
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-25
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The interaction between three flavonoids, i.e., Luteolin (LTL), Quercetin (QCT), and Naringenin (NGN) and bovine lactoferrin (BLF) at pH 7.4 was investigated by fluorescence quenching spectra, synchronous fluorescence spectra, and UV-visible absorption spectra.
The results indicate the fluorescence of BLF quenched by Luteolin (LTL), Quercetin (QCT), and Naringenin (NGN) via static quenching.
The main force between QCT and LTL with BLF was van der Waals interactions and hydrogen bonds.
Electrostatic interactions played a major role in the binding process of interaction between NGN and BLF.
Synchronous fluorescence was used to study the conformational changes of BLF.
The values of binding constant (Ka) and number of binding sites (n) at different temperatures (300K, 305K, 310K) were also calculated, respectively.
The results of corresponding thermodynamic parameters as well as binding distance between BLF and LTL, QCT, or GNG were obtained.
These results implied that Luteolin (LTL), Quercetin (QCT), and Naringenin (NGN) could provide important guides for compound quantity (e.g., medicine dosage) and the design of new compounds (or drugs).
American Psychological Association (APA)
Huang, Junyi& Liu, Zhenmin& Ma, Qiaorong& He, Ziyu& Niu, Zhidian& Zhang, Mengying…[et al.]. 2018. Studies on the Interaction between Three Small Flavonoid Molecules and Bovine Lactoferrin. BioMed Research International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1128582
Modern Language Association (MLA)
Huang, Junyi…[et al.]. Studies on the Interaction between Three Small Flavonoid Molecules and Bovine Lactoferrin. BioMed Research International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1128582
American Medical Association (AMA)
Huang, Junyi& Liu, Zhenmin& Ma, Qiaorong& He, Ziyu& Niu, Zhidian& Zhang, Mengying…[et al.]. Studies on the Interaction between Three Small Flavonoid Molecules and Bovine Lactoferrin. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1128582
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128582