Kinetics of Targeted Phage Rescue in a Mouse Model of Systemic Escherichia coli K1
Joint Authors
Kovács, Tamás
Dorn, Á.
Cox, A.
Szentes, Nikolett
Horváth, Marianna
Nagy, Gábor
Doffkay, Zsolt
Maróti, Gergely
Rákhely, Gábor
Schneider, György
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Escherichia (E.) coli K1 strains remain common causative agents of neonatal sepsis and meningitis.
We have isolated a lytic bacteriophage (ΦIK1) against E.
coli strain IHE3034 and tested its specificity in vitro, as well as distribution and protective efficacy in vivo.
The phage was shown to be specific to the K1 capsular polysaccharide.
In the lethal murine model, a high level of protection was afforded by the phage with strict kinetics.
A single dose of 1 x 108 phage particles administered 10 and 60 minutes following the bacterial challenge elicited 100 % and 95 % survival, respectively.
No mice could be rescued if phage administration occurred 3 hours postinfection.
Tissue distribution surveys in the surviving mice revealed that the spleen was the primary organ in which accumulation of active ΦIK1 phages could be detected two weeks after phage administration.
These results suggest that bacteriophages have potential as therapeutic agents in the control of systemic infections.
American Psychological Association (APA)
Schneider, György& Szentes, Nikolett& Horváth, Marianna& Dorn, Á.& Cox, A.& Nagy, Gábor…[et al.]. 2018. Kinetics of Targeted Phage Rescue in a Mouse Model of Systemic Escherichia coli K1. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1128605
Modern Language Association (MLA)
Schneider, György…[et al.]. Kinetics of Targeted Phage Rescue in a Mouse Model of Systemic Escherichia coli K1. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1128605
American Medical Association (AMA)
Schneider, György& Szentes, Nikolett& Horváth, Marianna& Dorn, Á.& Cox, A.& Nagy, Gábor…[et al.]. Kinetics of Targeted Phage Rescue in a Mouse Model of Systemic Escherichia coli K1. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1128605
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128605