Potential of Maintaining a Healthy Vaginal Environment by Two Lactobacillus Strains Isolated from Cocoa Fermentation
Joint Authors
Passos Rezende, Rachel
Romano, Carla Cristina
Melgaço, Ana Clara Correia
Blohem Pessoa, Wallace Felipe
Freire, Herbert Pina
Evangelista de Almeida, Milena
Santos Barbosa, Maysa
Timenetsky, Jorge
Miranda Marques, Lucas
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-30
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Bacteria in the genera Mycoplasma and Ureaplasma do not have cell walls and therefore interact with host cells through lipid-associated membrane proteins (LAMP).
These lipoproteins are important for both surface adhesion and modulation of host immune responses.
Mycoplasma and Ureaplasma have been implicated in cases of bacterial vaginosis (BV), which can cause infertility, abortion, and premature delivery.
In contrast, bacteria of the genus Lactobacillus, which are present in the vaginal microbiota of healthy women, are thought to inhibit local colonization by pathogenic microorganisms.
The aim of the present study was to evaluate the in vitro interactions between lipoproteins of Mycoplasma and Ureaplasma species and vaginal lineage (HMVII) cells and to study the effect of Lactobacillus isolates from cocoa fermentation on these interactions.
The tested Lactobacillus strains showed some important probiotic characteristics, with autoaggregation percentages of 28.55% and 31.82% for L.
fermentum FA4 and L.
plantarum PA3 strains, respectively, and percent adhesion values of 31.66 and 41.65%, respectively.
The two strains were hydrophobic, with moderate to high hydrophobicity values, 65.33% and 71.12% for L.
fermentum FA4 and L.
plantarum PA3 in toluene.
Both strains secreted acids into the culture medium with pH=4.32 and pH=4.33, respectively, and showed antibiotics susceptibility profiles similar to those of other lactobacilli.
The strains were also able to inhibit the death of vaginal epithelial cells after incubation with U.
parvum LAMP from 41.03% to 2.43% (L.
fermentum FA4) and 0.43% (L.
plantarum PA3) and also managed to significantly decrease the rate of cell death caused by the interaction with LAMP of M.
hominis from 34.29% to 14.06% (L.
fermentum FA4) and 14.61% (L.
plantarum PA3), thus demonstrating their potential for maintaining a healthy vaginal environment.
American Psychological Association (APA)
Melgaço, Ana Clara Correia& Blohem Pessoa, Wallace Felipe& Freire, Herbert Pina& Evangelista de Almeida, Milena& Santos Barbosa, Maysa& Passos Rezende, Rachel…[et al.]. 2018. Potential of Maintaining a Healthy Vaginal Environment by Two Lactobacillus Strains Isolated from Cocoa Fermentation. BioMed Research International،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1128611
Modern Language Association (MLA)
Melgaço, Ana Clara Correia…[et al.]. Potential of Maintaining a Healthy Vaginal Environment by Two Lactobacillus Strains Isolated from Cocoa Fermentation. BioMed Research International No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1128611
American Medical Association (AMA)
Melgaço, Ana Clara Correia& Blohem Pessoa, Wallace Felipe& Freire, Herbert Pina& Evangelista de Almeida, Milena& Santos Barbosa, Maysa& Passos Rezende, Rachel…[et al.]. Potential of Maintaining a Healthy Vaginal Environment by Two Lactobacillus Strains Isolated from Cocoa Fermentation. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1128611
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128611