![](/images/graphics-bg.png)
Improvement of K562 Cell Line Transduction by FBS Mediated Attachment to the Cell Culture Plate
Joint Authors
Abbasalipour, Maryam
Khosravi, Mohammad Ali
Zeinali, Sirous
Khanahmad, Hossein
Azadmanesh, Kayhan
Karimipour, Morteza
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-03-27
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Lentiviral vectors have been used for gene therapy in the clinical phase in recent years.
These vectors provide a tool for gene insertion, deletion, or modification in organisms.
The K562 human cell line has been used extensively in hematopoietic research.
Despite its broad application, it is hard-to-transfection and transduction.
So, this study presents a simple method to increase the transduction efficiency of K562 cells with a low multiplicity of infection (MOI) of the virus particle.
For this purpose, 24-well plate was coated by 300 μl fetal bovine serum (FBS) before seeding.
Then 2×104 K562 cells were seeded in each FBS coated plate.
After 24h, K562 cells were attached and doubled.
Different amount of lentivirus-based GFP vector according to MOI (5, 10, 15, and 20) along with 8 μg polybrene was added to the attached K562 cells and after 6h cells and viral particle complex were spinfected.
Then cells were returned to the plate and incubated in 37°C overnight.
After 48h transduction efficiency was established by measuring the GFP-expressing cells by flow cytometry.
Flow cytometry analysis showed that, after plate treatment by FBS, 64.5% transduction rate in K562 cells was achieved at MOI=20.
Therefore, this method can be an effective and simple way to increase the lentiviral transduction rate for suspended cells such as K562.
American Psychological Association (APA)
Abbasalipour, Maryam& Khosravi, Mohammad Ali& Zeinali, Sirous& Khanahmad, Hossein& Karimipour, Morteza& Azadmanesh, Kayhan. 2019. Improvement of K562 Cell Line Transduction by FBS Mediated Attachment to the Cell Culture Plate. BioMed Research International،Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1128624
Modern Language Association (MLA)
Abbasalipour, Maryam…[et al.]. Improvement of K562 Cell Line Transduction by FBS Mediated Attachment to the Cell Culture Plate. BioMed Research International No. 2019 (2019), pp.1-6.
https://search.emarefa.net/detail/BIM-1128624
American Medical Association (AMA)
Abbasalipour, Maryam& Khosravi, Mohammad Ali& Zeinali, Sirous& Khanahmad, Hossein& Karimipour, Morteza& Azadmanesh, Kayhan. Improvement of K562 Cell Line Transduction by FBS Mediated Attachment to the Cell Culture Plate. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-6.
https://search.emarefa.net/detail/BIM-1128624
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128624