T m -Shift Detection of Dog-Derived Ancylostoma ceylanicum and A. caninum
Joint Authors
Li, Guoqing
Abullahi, Auwalu Yusuf
Fu, Yeqi
Wang, Mingwei
Yan, Xinxin
Hang, Jianxiong
Zhang, Pan
Huang, Yue
Liu, Yunqiu
Sun, Yongxiang
Ran, Rongkun
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
To develop a Tm-shift method for detection of dog-derived Ancylostoma ceylanicum and A.
caninum, three sets of primers were designed based on three SNPs (ITS71, ITS197, and ITS296) of their internal transcribed spacer 1 (ITS1) sequences.
The detection effect of the Tm-shift was assessed through the stability, sensitivity, accuracy test, and clinical detection.
The results showed that these three sets of primers could distinguish accurately between A.
ceylanicum and A.
caninum.
The coefficient of variation in their Tm values on the three SNPs was 0.09% and 0.15% (ITS71), 0.18% and 0.14% (ITS197), and 0.13% and 0.07% (ITS296), respectively.
The lowest detectable concentration of standard plasmids for A.
ceylanicum and A.
caninum was 5.33 × 10−6 ng/μL and 5.03 × 10−6 ng/μL.
The Tm-shift results of ten DNA samples from the dog-derived hookworms were consistent with their known species.
In the clinical detection of 50 fecal samples from stray dogs, the positive rate of hookworm detected by Tm-shift (42%) was significantly higher than that by microscopic examination (34%), and the former can identify the Ancylostoma species.
It is concluded that the Tm-shift method is rapid, specific, sensitive, and suitable for the clinical detection and zoonotic risk assessment of the dog-derived hookworm.
American Psychological Association (APA)
Fu, Yeqi& Wang, Mingwei& Yan, Xinxin& Abullahi, Auwalu Yusuf& Hang, Jianxiong& Zhang, Pan…[et al.]. 2018. T m -Shift Detection of Dog-Derived Ancylostoma ceylanicum and A. caninum. BioMed Research International،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1128630
Modern Language Association (MLA)
Fu, Yeqi…[et al.]. T m -Shift Detection of Dog-Derived Ancylostoma ceylanicum and A. caninum. BioMed Research International No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1128630
American Medical Association (AMA)
Fu, Yeqi& Wang, Mingwei& Yan, Xinxin& Abullahi, Auwalu Yusuf& Hang, Jianxiong& Zhang, Pan…[et al.]. T m -Shift Detection of Dog-Derived Ancylostoma ceylanicum and A. caninum. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1128630
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128630