Chloroquine Protects Human Corneal Epithelial Cells from Desiccation Stress Induced Inflammation without Altering the Autophagy Flux
Joint Authors
Shetty, Rohit
Ghosh, Arkasubhra
Shivakumar, Shivapriya
Panigrahi, Trailokyanath
Subramani, Murali
Jeyabalan, Nallathambi
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-01
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Dry eye disease (DED) is a multifactorial ocular surface disorder affecting millions of individuals worldwide.
Inflammation has been associated with dry eye and anti-inflammatory drugs are now being targeted as the alternate therapeutic approach for dry eye condition.
In this study, we have explored the anti-inflammatory and autophagy modulating effect of chloroquine (CQ) in human corneal epithelial and human corneal fibroblasts cells exposed to desiccation stress, (an in-vitro model for DED).
Gene and protein expression profiling of inflammatory and autophagy related molecular factors were analyzed in HCE-T and primary HCF cells exposed to desiccation stress with and without CQ treatment.
HCE-T and HCF cells exposed to desiccation stress exhibited increased levels of activated p65, TNF-α, MCP-1, MMP-9, and IL-6.
Further, treatment with CQ decreased the levels of active p65, TNF-α, MCP-1, and MMP-9 in cells underdesiccation stress.
Increased levels of LC3B and LAMP1 markers in HCE-T cells exposed to desiccation stress suggest activation of autophagy and the addition of CQ did not alter these levels.
Changes in the phosphorylation levels of MAPKinase and mTOR pathway proteins were found in HCE-T cells under desiccation stress with or without CQ treatment.
Taken together, the data suggests that HCE-T cells under desiccation stress showed NFκB mediated inflammation, which was rescued through the anti-inflammatory effect of CQ without altering the autophagy flux.
Therefore, CQ may be used as an alternate therapeutic management for dry eye condition.
American Psychological Association (APA)
Shivakumar, Shivapriya& Panigrahi, Trailokyanath& Shetty, Rohit& Subramani, Murali& Ghosh, Arkasubhra& Jeyabalan, Nallathambi. 2018. Chloroquine Protects Human Corneal Epithelial Cells from Desiccation Stress Induced Inflammation without Altering the Autophagy Flux. BioMed Research International،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1128644
Modern Language Association (MLA)
Shivakumar, Shivapriya…[et al.]. Chloroquine Protects Human Corneal Epithelial Cells from Desiccation Stress Induced Inflammation without Altering the Autophagy Flux. BioMed Research International No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1128644
American Medical Association (AMA)
Shivakumar, Shivapriya& Panigrahi, Trailokyanath& Shetty, Rohit& Subramani, Murali& Ghosh, Arkasubhra& Jeyabalan, Nallathambi. Chloroquine Protects Human Corneal Epithelial Cells from Desiccation Stress Induced Inflammation without Altering the Autophagy Flux. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1128644
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128644