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Tripartite Motif Containing 11 Interacts with DUSP6 to Promote the Growth of Human Osteosarcoma Cells through Regulating ERK12 Pathway
Joint Authors
Wang, Zhaofeng
Xu, Xiaobo
Tang, Wenxiao
Zhu, Youcai
Hu, Jichao
Zhang, Xingen
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Tripartite Motif Containing 11 (TRIM11), an E3 ubiquitin ligase, is identified as a carcinogen causing certain human cancers.
However, the specific role of TRIM11 is still uncovered in human osteosarcoma (OS) cells.
To explore the role of TRIM11 in OS cells, TRIM11 was induced by silencing and overexpression in OS cells using RNA interference (RNAi) and lentiviral vector, respectively.
qRT-PCR and western blot were used to examine the transcription and translation levels of the target gene.
Cell count kit-8 (CCK-8) assays were established to analyze cell proliferation.
Cell apoptosis ratio was determined via flow cytometry.
In our analyses, TRIM11 was suggested to be upregulated, and it functioned as a pro-proliferation and antiapoptosis factor in OS cells.
Moreover, the extracellular-signal-regulated kinase 1/2 (ERK1/2) inhibitor PD98059 was used to examine the relationship between TRIM11 and ERK1/2 in OS cells.
Results demonstrated that the role of TRIM11 was significantly disrupted by the ERK1/2 inhibitor PD98059.
Interestingly, we found TRIM11 overexpression did not affect dual-specificity phosphatase 6 (DUSP6) transcription, but improved its translation in OS cells.
Co-immunoprecipitation (Co-IP) analyses revealed that TRIM11 interacted with DUSP6.
Importantly, overexpression of TRIM11 enhanced DUSP6 ubiquitination in OS cells.
Therefore, TRIM11 might suppress the translation of DUSP6 via improving its ubiquitination.
Additionally, TRIM11 silencing in OS cells significantly reduced its tumorigenicity in vivo.
Overall, our findings firstly revealed that TRIM11 was an oncogene gene in the growth of OS cells and illustrated its potential function as a target in the treatment of OS.
American Psychological Association (APA)
Wang, Zhaofeng& Xu, Xiaobo& Tang, Wenxiao& Zhu, Youcai& Hu, Jichao& Zhang, Xingen. 2019. Tripartite Motif Containing 11 Interacts with DUSP6 to Promote the Growth of Human Osteosarcoma Cells through Regulating ERK12 Pathway. BioMed Research International،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1128673
Modern Language Association (MLA)
Wang, Zhaofeng…[et al.]. Tripartite Motif Containing 11 Interacts with DUSP6 to Promote the Growth of Human Osteosarcoma Cells through Regulating ERK12 Pathway. BioMed Research International No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1128673
American Medical Association (AMA)
Wang, Zhaofeng& Xu, Xiaobo& Tang, Wenxiao& Zhu, Youcai& Hu, Jichao& Zhang, Xingen. Tripartite Motif Containing 11 Interacts with DUSP6 to Promote the Growth of Human Osteosarcoma Cells through Regulating ERK12 Pathway. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1128673
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1128673